Various spider species produce dragline silks with different mechanical properties. The primary structure of silk proteins is thought to contribute to the elasticity and strength of the fibres. Previously published work has demonstrated that the dragline silk of Euprosthenops sp. is stiffer then comparable silk of Nephila edulis, Araneus diadematus and Latrodectus mactans. Our studies of Euprosthenops dragline silk at the molecular level have revealed that nursery web spider fibroin has the highest polyalanine content among previously characterised silks and this is likely to contribute to the superior qualities of pisaurid dragline.
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http://dx.doi.org/10.1016/j.cbpc.2004.04.020 | DOI Listing |
Biomacromolecules
January 2025
Shanghai Engineering Research Center of Molecular Therapeutics & New Drug Development, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, China.
Spider silk is renowned for its exceptional toughness, with the strongest dragline silk composed of two proteins, MaSp1 and MaSp2, featuring central repetitive sequences and nonrepetitive terminal domains. Although these sequences to spider silk's strength and toughness, the specific roles of MaSp1 and MaSp2 at the atomic level remain unclear. Using AlphaFold3 models and molecular dynamics (MD) simulations, we constructed models of MaSp1 and MaSp2 and validated their stability.
View Article and Find Full Text PDFAdv Healthc Mater
December 2024
Department of Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong SAR, 999077, China.
The advent of bionic skin sensors represents a significant leap forward in the realm of wearable health monitoring technologies. Existing bionic skin technologies face several limitations, including complex and expensive manufacturing processes, low wearing comfort, and challenges in achieving comfortable real-time health monitoring. These shortcomings hinder the widespread adoption and practical utility of bionic skin in various applications.
View Article and Find Full Text PDFCommun Chem
November 2024
Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
Liquid-liquid phase separation (LLPS) of proteins can be considered an intermediate solubility regime between disperse solutions and solid fibers. While LLPS has been described for several pathogenic amyloids, recent evidence suggests that it is similarly relevant for functional amyloids. Here, we review the evidence that links spider silk proteins (spidroins) and LLPS and its role in the spinning process.
View Article and Find Full Text PDFCarbohydr Polym
January 2025
The First Dongguan Affiliated Hospital, School of Pharmacy, Guangdong Medical University, Dongguan, 523808, China. Electronic address:
Emerging research attentions are focused on the development of fluorescent biomaterials for various biomedical applications, including fluorescence-guided surgery. However, it is still challenging to prepare biomolecules-based fluorescent fibers with both satisfactory biocompatibility and optimal mechanical properties. Here, we develop a fluorescent robust biofiber through using a tetraphenylethene-containing surfactant as the contact points between polysaccharide chains of alginate.
View Article and Find Full Text PDFAdv Mater
December 2024
Key Laboratory of Organic Optoelectronics and Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing, 100084, China.
High-performance silk fibers, with their eco-friendly degradability and renewability, have long captivated researchers as an alternative to synthetic fibers. Spider dragline silk, renowned for its exceptional strength (>1 GPa), has an extremely low yield, hindering its widespread use. While domesticated silkworms (Bombyx mori) can produce silk fibers industrially, their moderate strength (≈0.
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