AI Article Synopsis
- The study investigated the distribution and colocalization of HLA I, HLA-DR, and ICAM-1 proteins in the plasma membrane of two human uveal melanoma cell lines for the first time.
- The experiments revealed that these proteins are mostly located in the same membrane regions, forming similar patterns and associations as seen in other cell types.
- Additionally, the presence of IFN-gamma significantly increased these protein levels and modified their organization, potentially influencing the immune response from effector cells.
Article Abstract
The lateral distribution and colocalization of HLA I, HLA-DR, and ICAM-1 proteins was studied for the first time in the plasma membrane of two human uveal melanoma cell lines, OCM-1 and OCM-3. Our fluorescence resonance energy transfer and confocal laser scanning microscopic experiments revealed that these molecules are mostly confined to the same membrane regions, where they form similar protein patterns (homo- and hetero-associates) to those found previously on other cell types of lymphoid as well as colorectal carcinoma origin. Confocal microscopic colocalization experiments with GM(1) gangliosides and the GPI-anchored CD59 molecules showed enrichment of HLA I, HLA-DR, and ICAM-1 molecules in specific membrane domains (lipid rafts) excluding the transferrin receptor. IFN-gamma remarkably increased the expression levels of these molecules and rearranged their association patterns, which can affect the adoptive immune response of effector cells.
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| http://dx.doi.org/10.1016/j.bbrc.2004.07.171 | DOI Listing |
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