AI Article Synopsis
- A new rapid LC-MS/MS method was created to measure dopamine, 5-hydroxytryptamine, and norepinephrine in brain microdialysates.
- This method also allows for the simultaneous detection of these neurotransmitters alongside cocaine in brain samples.
- The entire analysis can be completed in just 4 minutes with specific detection limits for each substance specified (DA at 200 pM, NE at 1000 pM, 5HT at 900 pM, and cocaine at 1 pg/ml).
Article Abstract
A rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed for the measurement of dopamine (DA), 5-hydroxytryptamine (5HT) and norepinephrine (NE) in brain microdialysates. The assay has also been utilised for the simultaneous measurement of these neurotransmitters and cocaine in brain dialysates. The neurotransmitters and cocaine were resolved in a single 4-min run using a binary gradient elution profile. The analytes were detected using tandem mass spectrometry in the positive ion electrospray mode. The limits of detection for DA, NE, 5HT and cocaine were 200, 1000, 900 pM and 1 pg ml(-1), respectively.
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| http://dx.doi.org/10.1016/j.jneumeth.2004.03.021 | DOI Listing |
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