Large unilamellar vesicles of egg-phosphatidylcholine (eggPC), a naturally occurring phospholipid, were used in capillary electrophoresis (CE) for semi-permanent coating of fused silica capillaries. The stability of the phospholipid coating was tested at different cetyltrimethylammonium bromide (CTAB) concentrations with and without CaCl(2) present in the coating solution. The effect of physical factors influencing the coating stability (e.g. duration of the coating time, storage temperature of the coating solution) were also studied. Standing overnight in background electrolyte (BGE) solution did not alter the eggPC phospholipid coating noticeably. The performance of the coating was tested with a mixture of basic proteins (lysozyme, ribonuclease A and alpha-chymotrypsinogen A). Highest efficiencies (over 200,000 plates m(-1)) were achieved when the capillary was filled for 15 h with a liposome solution containing both CTAB and CaCl(2).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00216-004-2722-y | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Constructing Activatable Photosensitizers Using Covalently Modified Mesoporous Silica.
Adv Sci (Weinh)
January 2025
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry and Chemical Engineering, Donghua University, Shanghai, 201620, China.
The combination of photosensitizers (PSs) and nanomaterials is a widely used strategy to enhance PS efficacy and broaden their applicability. However, the current nanocarrier-based delivery strategies focus on conventional PSs, neglecting the critical issue of PS phototoxicity. In this study, DHUOCl-25, an activatable PS (aPS) activated by hypochlorous acid, is synthesized by combining a silicon source structure and an activation unit.
View Article and Find Full Text PDFFacile Synthesis of Monodisperse Gold Nanorods, Gold Nanobipyramids and Gold Nanocups with Different Coatings and Evaluation of Their Cellular Cytotoxicity.
Curr Drug Deliv
January 2025
School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
Article Synopsis
- The study highlights the rising importance of assessing the cytotoxicity of gold nanoparticles (GNPs) in biomedical applications.
- Researchers synthesized three types of GNPs—gold nanorods (GNRs), gold nanobipyramids (GNBPs), and gold nanocups (GNCs)—using a specific method and measured their sizes.
- The experiments showed that the cytotoxic effects of GNPs varied based on their shape and surface coating, with CTAB-coated GNPs being more harmful than PEG-coated ones, indicating that these factors significantly influence GNP behavior in cells.
Environmentally tolerant multifunctional eutectogel for highly sensitive wearable sensors.
Mater Horiz
January 2025
Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong University, Chengdu 610031, China.
Article Synopsis
- Flexible hydrogel sensors have limitations in sensitivity and freezing in low temperatures, hindering their applications.
- A new multifunctional eutectogel is developed through photopolymerization, offering properties like high transparency, anti-freezing, and self-healing.
- This eutectogel shows exceptional performance with a high gauge factor for strain sensitivity, making it promising for flexible electronics in cold conditions.
Thermal Transport through CTAB- and MTAB-Functionalized Gold Interfaces Using Molecular Dynamics Simulations.
J Chem Inf Model
January 2025
251 Nieuwland Science Hall, Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, United States.
Thermal transport coefficients, notably the interfacial thermal conductance, were determined in planar and spherical gold interfaces functionalized with CTAB (cetyltrimethylammonium bromide) or MTAB (16-mercapto-hexadecyl-trimethylammonium bromide) using reverse nonequilibrium molecular dynamics (RNEMD) methods. The systems of interest included (111), (110), and (100) planar facets as well as nanospheres ( = 10 Å). The effect of metal polarizability was investigated through the implementation of the density-readjusted embedded atom model (DR-EAM), a polarizable metal potential.
View Article and Find Full Text PDFStudy on Water Damage of Asphalt-Aggregate Based on Molecular Dynamics.
Materials (Basel)
January 2025
College of Civil Engineering and Architecture, Shandong University of Science and Technology, Qingdao 266590, China.
To investigate the water damage at the interface between emulsified asphalt and aggregate under the action of external water infiltration, firstly, cetyltrimethylammonium bromide was used as an emulsifier to prepare emulsified asphalt in the laboratory, and its basic properties were tested. Then, based on molecular dynamics, an emulsified asphalt-aggregate interface model with different water contents was constructed to calculate the adhesion work of the emulsified asphalt-aggregate interface. The results show that the simulated values of emulsified asphalt density, cohesive energy density, and solubility are in good agreement with the experimental values.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!