Background: In lung transplant recipients (LTRs), the measurement of human cytomegalovirus (HCMV) load dynamics in the local environment of the lung allograft may offer distinct advantages over their assessment in the peripheral blood compartment.
Methods: We compared HCMV load in paired bronchoalveolar lavage (BAL) and plasma samples in a prospective cohort of LTRs.
Results: In all, 182 paired samples were collected from 41 LTRs. Qualitative findings were concordant for 79.7% of the paired samples (virus load detected or absent in 8.1% and 71.5% of paired samples, respectively). In 35 paired samples (20.3%), HCMV was detected in the BAL but not the plasma sample; the converse was not found for any of the paired samples. HCMV inclusions were histologically detected in the lungs of 8 patients on 9 occasions. HCMV was detected in both BAL and plasma samples on 7 of the 9 occasions and in only the BAL sample on 2 occasions. Application of a threshold virus load in BAL samples, to predict histologically proven HCMV infection, was associated with improved diagnostic sensitivity and specificity.
Conclusions: Quantification of HCMV in the lung allograft correlates with histological detection and, in addition, offers the potential to monitor subclinical viral replication in the lung allograft.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1086/422327 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development and utilization of a multiplex PCR assay for detecting three feline enteroviruses.
Mol Biol Rep
January 2025
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, PR China.
Background: Feline diarrhea is a common digestive tract disease in clinical practice, with watery feces as the main clinical manifestation. There are numerous pathogenic factors causing feline diarrhea, among which viral infections are prevalent, and feline panleukopenia virus (FPV) is the most common pathogen. In recent years, a variety of novel viruses have been detected in the intestines of cats with diarrhea.
View Article and Find Full Text PDFBetween-day variability of soleus H reflex recruitment curve parameters: how many stimulations are required to maximise the reproducibility of the measurements?
Somatosens Mot Res
January 2025
Neuromuscular Research Lab, Faculdade de Motricidade Humana, Universidade de Lisboa, Estrada da Costa, Dafundo, Portugal.
Purpose: The H reflex recruitment curve represents the gold standard for quantifying changes in spinal circuitries. However, there is no agreement on how many stimulations should be applied for each parameter. Thus, we explored the impact of varying the number of stimulations (3, 6, 9, 12 and 15 stimuli per intensity) on between-day reliability of soleus H reflex.
View Article and Find Full Text PDFIdentification of mitophagy-related key genes and their correlation with immune cell infiltration in acute myocardial infarction via bioinformatics analysis.
Front Cardiovasc Med
January 2025
Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, China.
Background: Acute myocardial infarction (AMI), a subset of acute coronary syndrome, remains the major cause of mortality worldwide. Mitochondrial dysfunction is critically involved in AMI progression, and mitophagy plays a vital role in eliminating damaged mitochondria. This study aimed to explore mitophagy-related biomarkers and their potential molecular basis in AMI.
View Article and Find Full Text PDFComputational discovery of co-expressed antigens as dual targeting candidates for cancer therapy through bulk, single-cell, and spatial transcriptomics.
Bioinform Adv
June 2024
Department of Pediatrics and Human Development, Michigan State University, Grand Rapids, MI 49503, United States.
Motivation: Bispecific antibodies (bsAbs) that bind to two distinct surface antigens on cancer cells are emerging as an appealing therapeutic strategy in cancer immunotherapy. However, considering the vast number of surface proteins, experimental identification of potential antigen pairs that are selectively expressed in cancer cells and not in normal cells is both costly and time-consuming. Recent studies have utilized large bulk RNA-seq databases to propose bispecific targets for various cancers.
View Article and Find Full Text PDFA New Anatomical Approach to Male Pectoral Volumization.
Plast Reconstr Surg Glob Open
January 2025
Department of Plastic and Reconstructive Surgery, Acute Surgical Pathology Clinic Hospital General de México, Dr Eduardo Liceaga, Mexico City, Mexico.
Background: In this article, we discuss a new technique of pectoralis major muscle volumization using ultrasound-guided fat grafting, considering a new surgical criterion that allows for larger volumes.
Methods: We present a retrospective observational descriptive study, which included men who underwent body contouring surgery during 2022-2023. As part of the study, measurements were taken before, immediately after, and at 3 months after surgery.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!