Objective: To investigate the transcriptions of 18 chemokine receptors in human decidual natural killer (NK) cells and explore the possible mechanisms of preferential accumulation of CD56(bright)CD16(-)NK cells in decidua during first-trimester pregnancy.
Methods: Villi and decidual tissue were collected from normal pregnant women with 5 approximately 10 gestational weeks by artificial abortion. The decidual CD56(bright)CD16(-)NK cells were isolated by immune magnetic beads. The transcription levels of 18 chemokine receptors in the decidual CD56(bright)CD16(-)NK cells were assessed by reverse transcription-polymerase chain reaction (RT-PCR). After the high expression of CXCR4 and CXCR3 mRNA in these cells was found, the expression of stromal cell-derived factor-1 (SDF-1), the specific ligand of CXCR4, in first-trimester human placenta was detected by in situ hybridization and immunohistochemistry. The concentration of SDF-1alpha in the supernatant of culture of isolated trophoblast cells derived from the first-trimester human placentas was measured by ELISA. The chemotaxis of SDF-1 to decidual CD56(bright)CD16(-)NK cells was tested in Transwell, and the chemotactic activity was quantitatively examined.
Results: Among the 18 chemokine receptors, CXCR4 and CXCR3 were found highly transcribed in decidual CD56(bright)CD16(-)NK cells. The concentration of SDF-1alpha in the supernatant was 385 ng/L +/- 91 ng/L after trophoblast cells had been cultured for 60 hours. Both rhSDF-1alpha and supernatants in the culture of trophoblast cells exhibited chemotactic activity on decidual CD56(bright)CD16(-)NK cells. When the concentration of rhSDF-1alpha was 10 micro g/L the number of cells that entered the lower chamber of Transwell accounted for 22.9% +/- 4.3% of the total calls.
Conclusion: First-trimester human trophoblast cells produce SDF-1, which in turn endows the trophoblast cells with the capacity to attract decidual CD56(bright)CD16(-)NK cells highly expressing CXCR4. This activity contributes to the recruitment of decidual lymphocytes and may be used at a local level to manipulate the microimmune environment at the materno-fetal interface.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Immune cell activity during anti-TNF treatment in patients with psoriasis and psoriatic arthritis.
Clin Exp Immunol
November 2024
Broegelmann Research Laboratory, Department of Clinical Science, University of Bergen, Bergen, Norway.
Psoriasis is a chronic, inflammatory skin disease characterized by a dysregulated immune response and systemic inflammation. Up to one-third of patients with psoriasis have psoriatic arthritis (PsA). Targeted treatment with antibodies neutralizing tumor necrosis factor can ameliorate both diseases.
View Article and Find Full Text PDFCD56brightCD16- to CD57+CD56dimCD16+ NK cell ratio discriminates disease activity and renal involvement in patients with systemic lupus erythematosus.
Clin Exp Rheumatol
September 2023
School of Medicine, Southeast University, Nanjing; and Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
Objectives: We aimed to discriminate subpopulations of peripheral natural killer (NK) cells of patients with systemic lupus erythematosus (SLE) and evaluate their usability in monitoring disease activity.
Methods: The total number of NK cells and their subpopulations were determined by flow cytometry in 68 patients with SLE and 35 healthy controls. Clinical data were extracted from medical records, including serum anti-double-stranded-DNA (anti-dsDNA), complement C3 and C4, and urine protein.
Natural killer cell profiles in recurrent pregnancy loss: Increased expression and positive associations with TACTILE and LILRB1.
Am J Reprod Immunol
November 2022
Department of Transplantation Immunology, Maastricht University Medical Centre, Maastricht, the Netherlands.
Problem: NK cells are important for healthy pregnancy and aberrant phenotypes or effector functions have been associated with RPL. We compared expression of a broad panel of NK cell receptors, including immune checkpoint receptors, and investigated their clinical association with RPL as this might improve patient stratification and prediction of RPL.
Method Of Study: Peripheral blood mononuclear cells were isolated from 52 women with RPL and from 2 women with an uncomplicated pregnancy for flowcytometric analysis and plasma was used to determine anti-CMV IgG antibodies.
Extensive activation, tissue trafficking, turnover and functional impairment of NK cells in COVID-19 patients at disease onset associates with subsequent disease severity.
PLoS Pathog
April 2021
Division of Infectious Diseases, Policlinico San Martino Hospital, Genoa, Italy.
The SARS-CoV-2 infection causes severe respiratory involvement (COVID-19) in 5-20% of patients through initial immune derangement, followed by intense cytokine production and vascular leakage. Evidence of immune involvement point to the participation of T, B, and NK cells in the lack of control of virus replication leading to COVID-19. NK cells contribute to early phases of virus control and to the regulation of adaptive responses.
View Article and Find Full Text PDFImpaired T- and NK-cell reconstitution after haploidentical HCT with posttransplant cyclophosphamide.
Blood Adv
January 2021
Department of Medical Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA.
Administration of posttransplant cyclophosphamide (PTCy) has significantly expanded the number of patients undergoing HLA-haploidentical hematopoietic cell transplantation (haplo-HCT). To examine immune reconstitution in these patients, we monitored T- and natural killer (NK)-cell recovery in 60 patients receiving bone marrow or peripheral blood stem cell (PBSC) grafts after haplo-HCT with PTCy and 35 patients receiving HLA-matched donor PBSC grafts with standard graft-versus-host disease (GVHD) prophylaxis. Compared with HLA-matched recipients, early T-cell recovery was delayed in haplo-HCT patients and skewed toward effector memory T cells with markedly reduced naive T cells.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!