AI Article Synopsis
- A new fiber optic biosensor method is introduced for detecting signaling molecules activated by trophic factors in cells, specifically focusing on phosphorylated STAT3 in neuroblastoma cells.
- The technique utilizes a single fiber-optic probe and a 405 nm diode laser to excite the analyte, combined with a charged coupled device (CCD) spectrometer to capture the fluorescence signal.
- This new method is significantly more sensitive, offering detection capabilities that are 100 times better than traditional Western blotting techniques.
Article Abstract
This paper describes a highly sensitive method to detect trophic factor activated signaling molecules in cells using a compact fiber optic biosensor. The method is demonstrated by quantitative detection of phosphorylation of signal transducers and activators of transcription 3 (STAT3) in neuroblastoma cells. A single fiber-optic probe based on total internal reflection fluorescence sensing system is used. A 405 nm diode laser is used for evanescent wave excitation of immobilized labelled analyte on the probe surface. A compact charged coupled device (CCD) based spectrometer is used for recording the fluorescence signal. The method is two orders of magnitude more sensitive than the Western blotting technique.
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| http://dx.doi.org/10.1016/j.bios.2004.02.004 | DOI Listing |
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