Bme585 I is a new member of the restriction endonuclease type IIS family. It was partially purified from the heterothrophic, mesophilic bacterial strain Bacillus mesentericus 585 by ammonium sulphate precipitation and phosphocellulose column chromatography. Bme585 I is a monomeric protein with a molecular mass of 62 kD. The enzyme is active over a broad pH range from 7.0 to 8.8, has a temperature optimum of 37 degrees C and tolerance of NaCl in reaction buffer from 0 to 400 mM. Bme585 I recognizes the asymmetric sequence 5'-CCCGC(4/6)-3' and is therefore an isoschizomer of restriction endonuclease Fau I.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.micres.2004.01.010DOI Listing

Publication Analysis

Top Keywords

restriction endonuclease
12
isoschizomer restriction
8
endonuclease fau
8
strain bacillus
8
bacillus mesentericus
8
bme585
4
bme585 [5'-cccgc4/6-3']
4
[5'-cccgc4/6-3'] isoschizomer
4
fau isolated
4
isolated strain
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!