AI Article Synopsis
- Three types of poly(ethylene-co-acrylic) acid copolymers were immobilized on silica to create stationary phases for chromatography, using different spacer molecules.
- The study observed that stationary phases with more rigid alkyl chain structures showed better selectivity for certain carotenoid isomers, like beta-carotene and xanthophylls.
- This research demonstrated that the separation of analytes was influenced by polar interactions and the conformation of alkyl chains, as evidenced by NMR spectroscopy results.
Article Abstract
Three poly(ethylene-co-acrylic) acid copolymers (-CH(2)CH(2)-)(x)[CH(2)CH(CO(2)H)-](y) with different chain lengths and mass fractions of acrylic acid were covalently immobilized as stationary phases on silica via two variants of spacer molecules (3-aminopropyltriethoxysilane and 3-glycidoxypropyltrimethoxysilane). Different mobilities of the alkyl chains in the stationary phases were observed using (13)C solid-state NMR spectroscopy. The stationary phases with more rigid trans-ordered alkyl chains had better selectivity for geometric beta-carotene and xanthophyll isomers (provitamin A derivatives). Also, all the separations of the analytes were affected by polar interactions with the chromatographic sorbent. This was further proved by separating more polar cis/trans retinoic acid isomers (vitamin A derivatives). (13)C high-resolution/magic-angle spinning (HR/MAS) NMR measurements of the chromatographic sorbents suspended in the mobile phase confirmed a dependence of molecular shape recognition ability on alkyl chain conformation.
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| http://dx.doi.org/10.1007/s00216-004-2752-5 | DOI Listing |
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