AI Article Synopsis
- Chemical derivatization of peptides enhances their ionization for mass spectrometry by using Fmoc groups attached to surfaces.
- Laser desorption photoionization combines 337-nm and 157.6-nm pulses to generate large peptide fragments that can be identified.
- Fmoc acts as an ionization tag, reducing the ionization potential and enabling the detection of various molecular species associated with surfaces.
Article Abstract
Chemical derivatization of peptides allows efficient F2 laser single photon ionization (SPI) of Fmoc-derivatized peptides covalently bound to surfaces. Laser desorption photoionization mass spectrometry using 337-nm pulses for desorption and 157.6-nm pulses for threshold SPI forms large ions identified as common peptide fragments bound to either Fmoc or the surface linker. Electronic structure calculations indicate the Fmoc label is behaving as an ionization tag for the entire peptide, lowering the ionization potential of the complex below the 7.87-eV photon energy. This method should allow detection of many molecular species covalently or electrostatically bound to surfaces.
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| http://dx.doi.org/10.1021/ac049434t | DOI Listing |
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