Fibronectin (Fn) plays an important role in the adhesive function of many cells including neutrophils (PMN). We examined the hypothesis that activated PMN develop binding sites for fibronectin which allows for the aggregation of contiguous PMN. Because PMN adhesive function is altered in acute burn injury, we also investigated the role of Fn in the aggregation of PMN from subjects with acute thermal injury. The chemotactic peptide, n-formylmethionyl leucyl phenylalanine, induced rapid binding of radioiodinated plasma Fn to PMN. Significant binding of Fn was detected as early as 30 sec poststimulus and maximal binding occurred at 5 min. Fn binding was only partially reversible and nonsaturable. The chemotactic peptide induced aggregation and binding of Fn to PMN with similar kinetics, concentration dependence, temperature, and cation requirements. In burn patients, PMN demonstrated a significant decrease in chemotactic peptide-induced aggregation which was associated with decreased binding of Fn. Alterations in the binding of Fn to PMN may be responsible, in part, for diminished aggregation responses of PMN in the early stages of thermal injury.
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http://dx.doi.org/10.1016/0022-4804(92)90037-z | DOI Listing |
Front Vet Sci
January 2025
Graduate School of Integrated Sciences for Life, Hiroshima University, Higashihiroshima, Hiroshima, Japan.
Early detection of bovine mastitis-causing pathogens is necessary for treatment. As culturing methods are time-consuming, a more rapid detection technique is required. This study investigated the sensitivity, specificity, and detection limit of Gram staining of milk precipitates (milk Gram stain, MGS) to detect bovine mastitis-causing pathogens in milk, as well as the potential of MGS to diagnose inflammation by counting polymorphonuclear leukocytes (PMN).
View Article and Find Full Text PDFComput Struct Biotechnol J
December 2024
Cancer Data Science Laboratory, National Cancer Institute, Bethesda, MD, USA.
Exosomal microRNAs (exomiRs) play a critical role in intercellular communication, especially in cancer, where they regulate key cellular processes like proliferation, angiogenesis, and metastasis, highlighting their significance as potential diagnostic and therapeutic targets. Here, we aimed to characterize the role of exomiRs, derived from seven cancer types (four cell lines and three tumors), in influencing the pre-metastatic niche (PMN). In each cancer type we extracted high confidence exomiRs (LogFC >= 2 in exosomes relative to control), their experimentally validated targets, and the enriched pathways among those targets.
View Article and Find Full Text PDFAnimals (Basel)
January 2025
Department of Obstetrics and Reproduction, Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece.
Olive cake was incorporated at a low inclusion rate (3.7%) into the rations of dairy cows through partial substitution of maize, and its effects on milk production, general health, and fertility traits were investigated. Multiparous purebred Holstein dairy cows (n = 148) were divided into two groups: a treated group (n = 86) and a control group (n = 62).
View Article and Find Full Text PDFVet Immunol Immunopathol
January 2025
Department of Internal Medicine, College of Veterinary Medicine and Animal Science, University of Sao Paulo, São Paulo, Brazil. Electronic address:
Waste milk (WM) is commonly used in calf feeding to reduce rearing costs; however, its effects on the innate immune response remain unexplored. Therefore, this study aimed to evaluate the effects of WM on the innate immune response and inflammatory profile of pre-weaned dairy calves. Thirty male Holstein calves were assigned to receive pasteurized waste milk (PWM), saleable milk (SM), and WM (n = 10 in each group).
View Article and Find Full Text PDFProc Natl Acad Sci U S A
January 2025
Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Southeast University, Nanjing 210096, China.
Heterogeneous roles of complement C3 have been implicated in tumor metastasis and are highly context dependent. However, the underlying mechanisms linking C3 to tumor metastasis remain elusive in renal cell carcinoma (RCC). Here, we demonstrate that C3 of RCC cell-derived extracellular vesicles (EVs) contributes to metastasis via polarizing tumor-associated macrophages (TAMs) into the immunosuppressive phenotype and recruiting polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs).
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