Using conventional whole-cell voltage-clamp recordings, we examined the 4-aminopyridine (4-AP)- and tetraethylammonium (TEA)-sensitive K(+) currents in the cochlear inner hair cells (IHCs) of guinea pigs. 4-AP-sensitive currents were activated slowly and sustained the same current level, whereas TEA-sensitive currents were activated rapidly, followed by inactivation. The inactivation time course of TEA-sensitive currents was voltage-dependent, becoming faster at more depolarized levels. The inactivation of TEA-sensitive currents almost recovered within 5 ms. 4-AP- and TEA-sensitive K(+) currents coexisted in the same IHC.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/03655230410017625 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Kv channels improve the temporal processing of auditory neurons in the cochlear nucleus.
J Physiol
December 2024
Department of Otolaryngology - Head and Neck Surgery, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.
Article Synopsis
- Kv channels contribute to the A-type current in bushy neurons of the cochlear nucleus, which regulates neuronal excitability and is essential for processing temporal information in hearing.
- Using Jingzhaotoxin-X (JZ-X), researchers discovered that blocking this current increased excitability in bushy neurons and affected their firing behavior during high-rate activity.
- Findings indicate that while A-type currents are important for maintaining temporal processing in aging bushy neurons, changes in TEA-sensitive Kv currents are more indicative of the altered neuronal properties contributing to age-related hearing loss.
Comparative study of ionic currents and exocytosis in hair cells of the basilar and amphibian papilla in bullfrogs.
Front Cell Neurosci
January 2023
ENT Institute and Department of Otorhinolaryngology, Eye and ENT Hospital, Fudan University, Shanghai, China.
Hearing organs in the peripheral of different vertebrate species are extremely diverse in shape and function. In particular, while the basilar papilla (BP) is elongated and covers the sounds of both low and high frequencies in turtles and birds, it is round and responds to high frequencies only in frogs, leaving the low frequencies to the amphibian papilla (AP). In this study, we performed patch-clamp recordings in hair cells of both hearing organs in bullfrogs and conducted a comparative study of their ionic currents and exocytosis.
View Article and Find Full Text PDFFurther Studies on the Role of BTBD9 in the Cerebellum, Sleep-like Behaviors and the Restless Legs Syndrome.
Neuroscience
November 2022
Norman Fixel Institute for Neurological Diseases, Department of Neurology, College of Medicine, University of Florida, Gainesville, FL, USA. Electronic address:
Genetic analyses have linked BTBD9 to restless legs syndrome (RLS) and sleep regulation. Btbd9 knockout mice show RLS-like motor restlessness. Previously, we found hyperactivity of cerebellar Purkinje cells (PCs) in Btbd9 knockout mice, which may contribute to the motor restlessness observed.
View Article and Find Full Text PDFAfterhyperpolarization potential modulated by local [K] in K diffusion-restricted extracellular space in the central clock of suprachiasmatic nucleus.
Biomed J
August 2023
Department of Physiology and Pharmacology, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Healthy Aging Research Center, Chang Gung University, Taoyuan, Taiwan; Neuroscience Research Center, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan. Electronic address:
Background: Intercellular coupling is essential for the suprachiasmatic nucleus (SCN) to serve as a coherent central clock. Synaptic release of neurotransmitters and neuropeptides is critical for synchronizing SCN neurons. However, intercellular coupling via non-synaptic mechanisms has also been demonstrated.
View Article and Find Full Text PDFElectrical and Immunohistochemical Properties of Cochlear Fibrocytes in 3D Cell Culture and in the Excised Spiral Ligament of Mice.
J Assoc Res Otolaryngol
April 2022
School of Life Sciences, Keele University, Stoke-on-Trent, ST5 5BG, UK.
Fibrocyte degeneration in the cochlear lateral wall is one possible pathology of age-related metabolic hearing loss (presbycusis). Within the lateral wall fibrocytes play a role in potassium recycling and maintenance of the endocochlear potential. It has been proposed that cell replacement therapy could prevent fibrocyte degeneration in the CD/1 mouse model of hearing loss.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!