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Construction and application of a promoter-trapping vector with methyl parathion hydrolase gene mpd as the reporter. | LitMetric
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Construction and application of a promoter-trapping vector with methyl parathion hydrolase gene mpd as the reporter.

Zhong-Li Cui Xiao-Zhou Zhang Zhong-Hui Zhang Shun-Peng Li

Biotechnol Lett

Department of Microbiology, MOA Key Laboratory of Microbiological Engineering of the Agricultural Environment, Nanjing Agricultural University, Nanjing 210095, P.R. China.

Published: July 2004

A facilitative and efficient promoter-trapping vector, pUC-mpd, was constructed with the promoterless methyl parathion hydrolase gene as the reporter. This reporter gene is easily used to clone promoters with different promoting strength on selective plates. Promoter regions of the ytkA and ywoF genes with strong promoting and signal peptide functions were cloned from the Bacillus subtilis 168 genomic promoter library with this vector.

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 Source
http://dx.doi.org/10.1023/B:BILE.0000035481.03854.41DOI Listing

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