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http://dx.doi.org/10.1128/JB.186.15.4855-4860.2004 | DOI Listing |
Commun Biol
January 2025
College of Life Sciences, Capital Normal University, Haidian District, Beijing, China.
Phragmites australis is a globally distributed grass species (Poaceae) recognized for its vast biomass and exceptional environmental adaptability, making it an ideal model for studying wetland ecosystems and plant stress resilience. However, genomic resources for this species have been limited. In this study, we assembled a chromosome-level reference genome of P.
View Article and Find Full Text PDFJ Neurosci
January 2025
Department of Physiology, University of Maryland School of Medicine, Baltimore, MD, USA
The cell adhesion molecule Leucine-Rich Repeat Transmembrane neuronal protein 2 (LRRTM2) is crucial for synapse development and function. However, our understanding of its endogenous trafficking has been limited due to difficulties in manipulating its coding sequence (CDS) using standard genome editing techniques. Instead, we replaced the entire LRRTM2 CDS by adapting a two-guide CRISPR knock-in method, enabling complete control of LRRTM2.
View Article and Find Full Text PDFProg Mol Biol Transl Sci
January 2025
Department of Pharmacy, Birla Institute of Technology and Science Pilani, Pilani Campus, Rajasthan, India. Electronic address:
Recent advances in CRISPR-Cas systems have revolutionised the study and treatment of kidney diseases, including acute kidney injury (AKI), chronic kidney disease (CKD), diabetic kidney disease (DKD), lupus nephritis (LN), and polycystic kidney disease (PKD). CRISPR-Cas technology offers precise and versatile tools for genetic modification in monogenic kidney disorders such as PKD and Alport syndrome. Recent advances in CRISPR technology have also shown promise in addressing other kidney diseases like AKI, CKD, and DKD.
View Article and Find Full Text PDFSci Rep
January 2025
ENET Centre, VSB-Technical University of Ostrava, Ostrava, 708 00, Czech Republic.
Identifying the parameters of a solar photovoltaic (PV) model optimally, is necessary for simulation, performance assessment, and design verification. However, precise PV cell modelling is critical for design due to many critical factors, such as inherent nonlinearity, existing complexity, and a wide range of model parameters. Although different researchers have recently proposed several effective techniques for solar PV system parameter identification, it is still an interesting challenge for researchers to enhance the accuracy of the PV system modelling.
View Article and Find Full Text PDFEpigenetics Chromatin
January 2025
Department of Molecular Biology, Semmelweis University, Budapest, Hungary.
DNA methylation, catalyzed by DNA methyltransferases (DNMT), plays pivotal role in regulating embryonic development, gene expression, adaption to environmental stress, and maintaining genome integrity. DNMT family consists of DNMT1, DNMT3A, DNMT3B, and the enzymatically inactive DNMT3L. DNMT3A and DNMT3B establish novel methylation patterns maintained by DNMT1 during replication.
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