The products from the enzymatic beta-D-galactopyranosylation of 1D-chiro-inositol, 1D-pinitol, 1D-3-O-allyl-4-O-methyl-chiro-inositol, 1D-3,4-di-O-methyl-chiro-inositol, 1L-chiro-inositol and myo-inositol in combined yields ranging from 46% to 64% have been obtained using the beta-galactosidase isolated from an anaerobic extreme thermophile, Thermoanaerobacter sp. strain TP6-B1 and p-nitrophenyl beta-D-galactopyranoside as the donor. Analysis of the products from these reactions reveals information about the acceptor preferences of the enzyme.
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http://dx.doi.org/10.1016/j.carres.2004.05.016 | DOI Listing |
Bioresour Technol
January 2025
Department of Civil and Environmental Engineering, 473 Via Ortega, Stanford University, Stanford, CA 94305, USA; Department of Chemical Engineering, 443 Via Ortega, Stanford University, Stanford, CA 94305, USA; Novo Nordisk Foundation CO(2) Research Center, Aarhus University, Gustav Wieds Vej 10C, Aarhus C DK-8000, Denmark. Electronic address:
Front Microbiol
August 2024
Department of Chemical Engineering, Loughborough University, Loughborough, United Kingdom.
Thermophilic acetogens are gaining recognition as potent microbial cell factories, leveraging their unique metabolic capabilities to drive the development of sustainable biotechnological processes. These microorganisms, thriving at elevated temperatures, exhibit robust carbon fixation abilities via the linear Wood-Ljungdahl pathway to efficiently convert C substrates, including syngas (CO, CO and H) from industrial waste gasses, into acetate and biomass via the central metabolite acetyl-CoA. This review summarizes recent advancements in metabolic engineering and synthetic biology efforts that have expanded the range of products derived from thermophilic acetogens after briefly discussing their autotrophic metabolic diversity.
View Article and Find Full Text PDFTrends Biotechnol
December 2024
Environmental Biotechnology Group, Department of Geosciences, University of Tübingen, 72074 Tübingen, Germany; AG Angenent, Max Planck Institute for Biology, Max Planck Ring 5, D-72076 Tübingen, Germany; Department of Biological and Chemical Engineering, Aarhus University, Gustav Wieds Vej 10D, 8000Aarhus C, Denmark; The Novo Nordisk Foundation CO(2) Research Center (CORC), Aarhus University, Gustav Wieds Vej 10C, 8000 Aarhus, C, Denmark; Cluster of Excellence - Controlling Microbes to Fight Infections, University of Tübingen, Auf der Morgenstelle 28, 72074 Tübingen, Germany. Electronic address:
We recently proposed a two-stage Power-to-Protein technology to produce microbial protein from renewable electric power and CO. Two stages were operated in series: Clostridium ljungdahlii in Stage A to reduce CO with H into acetate, and Saccharomyces cerevisiae in Stage B to utilize O and produce microbial protein from acetate. Renewable energy can be used to power water electrolysis to produce H and O.
View Article and Find Full Text PDFMicrobiol Resour Announc
October 2024
Foundation for Applied Molecular Evolution, Alachua, Florida, USA.
We present the draft genome of the bacterium strain AK152, a thermophilic, endospore-spore-forming, anaerobe isolated from a hot spring in Grensdalur, in Southwestern Iceland. This assembled genome will lay the foundation for identifying the carboxylic and amino acid fermentation pathways, suggesting biotechnological applications for this strain.
View Article and Find Full Text PDFMicrobiol Resour Announc
September 2024
Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada Campus Querétaro, Instituto Politécnico Nacional, Querétaro, Querétaro, Mexico.
The draft genome of CM-CNRG TB177 isolated from an oil reservoir in Mexico was determined and annotated. The organism is a thermophilic and strict anaerobe bacterium that produces rhamnolipids, using glucose as a carbon source. The predicted genome size is 2,496,169 bp and 2,550 genes.
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