AI Article Synopsis
- The text introduces a vector system that enables controlled and low-level protein expression in human cells, enhancing the purification process of natural protein complexes.
- This system utilizes two affinity purification tags, called the Sequential Peptide Affinity (SPA) system, which helps prevent overproduction of target proteins.
- Additionally, an adaptation of the SPA system is developed for effective purification and identification of protein complexes in E. coli and possibly other bacteria.
Article Abstract
A vector system is described that combines reliable, very low level, regulated protein expression in human cells with two affinity purification tags (Sequential Peptide Affinity, or SPA, system). By avoiding overproduction of the target protein, this system allows for the efficient purification of natural protein complexes and their identification by mass spectrometry. We also present an adaptation of the SPA system for the efficient purification and identification of protein complexes in E. coli and, potentially, other bacteria.
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| http://dx.doi.org/10.1021/pr034084x | DOI Listing |
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