AI Article Synopsis
- G-protein-coupled receptor mRNAs are often found at low levels, making them difficult to study in terms of where and how much they are expressed.
- In situ hybridization (ISH) and Northern blotting are effective techniques that help researchers locate mRNAs and analyze their expression patterns, with ISH providing both cellular localization and semi-quantitative data, while Northern blotting allows for the identification of genetic variants and analysis of multiple RNA types.
- The protocols outlined offer detailed, step-by-step guidance for performing these techniques and interpreting the resulting data.
Article Abstract
G-protein-coupled receptor mRNAs are expressed at low levels and therefore present a challenge for the study of their sites and levels of expression. In situ hybridization (ISH) and Northern blotting are powerful methods for the localization of mRNAs and the study of regulation of mRNA expression. ISH combines the power of precise cellular localization with the ability to perform semiquantitative analysis of the mRNA level, whereas Northern blotting has the ability to identify genetic splice variants, or to study multiple RNA molecules sequentially in the same tissue samples. These protocols give step-by-step instructions for the performance of these techniques, and the analysis of the data that can be obtained using them.
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| http://dx.doi.org/10.1385/1-59259-754-8:099 | DOI Listing |
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