AI Article Synopsis
- Saccharomyces cerevisiae Hop2 and Mnd1 are crucial proteins for meiosis, with mutations causing issues in chromosome interactions and recombination.
- Analyses of double mutants reveal that HOP2, MND1, and DMC1 operate in the same pathway to facilitate the close pairing of homologous chromosomes during meiosis.
- Biochemical experiments show that Hop2 and Mnd1 form a heterodimer that binds more effectively to double-stranded DNA and enhances Dmc1's strand assimilation activity, indicating their interdependence in meiotic DNA recombination.
Article Abstract
Saccharomyces cerevisiae Hop2 and Mnd1 are abundant meiosisspecific chromosomal proteins, and mutations in the corresponding genes lead to defects in meiotic recombination and in homologous chromosome interactions during mid-prophase. Analysis of various double mutants suggests that HOP2, MND1, and DMC1 act in the same genetic pathway for the establishment of close juxtaposition between homologous meiotic chromosomes. Biochemical studies indicate that Hop2 and Mnd1 proteins form a stable heterodimer with a higher affinity for double-stranded than single-stranded DNA, and that this heterodimer stimulates the strand assimilation activity of Dmc1 in vitro. Together, the genetic and biochemical results suggest that Hop2, Mnd1, and Dmc1 are functionally interdependent during meiotic DNA recombination.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC490024 | PMC |
| http://dx.doi.org/10.1073/pnas.0404195101 | DOI Listing |
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