This paper reports on the structural characteristics of microcontact printed oligo(ethylene glycol)-terminated alkanethiol layers, HS(CH2)15CONH-(CH2CH2O)6-H (hereafter EG6), on gold. Microwetting, contact angle goniometry, imaging null ellipsometry, and infrared reflection-absorption spectroscopy (IRAS) are used to characterize the printed EG6 layers, and the quality of these layers in terms of layer thickness and the crystallinity of the alkyl and ethylene glycol portions is compared with data obtained from analogous layers prepared by solution self-assembly. The outcome of the printing process is critically dependent on the experimental parameters used to prepare the patterns. It is found that high quality layers, consisting of densely packed all-trans alkyl chains terminated with relatively helical hexa(ethylene glycol) tails, are formed by inking the poly(dimethylsiloxane) (PDMS) stamp with a 1 mM EG6 solution and contacting it with gold for 15 min. The homogeneity of printed layers is not as good as the homogeneity of those prepared from solution under similar conditions, most likely because of simultaneous transfer of low molecular weight residues from the PDMS stamp. These residues, however, can be easily removed upon ultrasonication in ethanol without affecting the quality of the printed layer. Further on, the microscopic square-shaped bare gold patterns formed after microcontact printing (muCP) are subsequently filled with 16-hexadecanoic acid (hereafter THA) or HS(CH2)15CONH-(CH2CH2O)6-COOH (hereafter EG6COOH) to provide a microarray platform for further covalent attachment of biomolecules. Well-defined structures in terms of wettability contrast, sharpness, and height differences between the printed and back-filled areas are confirmed by imaging null ellipsometry and microscopic wetting.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/la049421a | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Silver Microdisc Array Electrode Chip for Urea Detection in Saliva Samples from Patients with Chronic Nephritis.
Anal Chem
January 2025
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Urea is an important biomarker for diagnosing various kidney and liver disorders. However, many existing methods rely on invasive blood sampling, which can potentially harm patients. Saliva has been recently recognized as a noninvasive and easily collectible alternative to blood for urea quantification.
View Article and Find Full Text PDFChromogenic Photonic Crystal Detectors for Monitoring Small Molecule Diffusion at Solid-Solid Interfaces Using Stimuli-Responsive Shape Memory Polymers.
ACS Appl Mater Interfaces
January 2025
Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, Florida 32611, United States.
In situ monitoring of small molecule diffusion at solid-solid interfaces is challenging, even with sophisticated equipment. Here, novel chromogenic photonic crystal detectors enabled by integrating bioinspired structural color with stimuli-responsive shape memory polymer (SMP) for detecting trace amounts of small molecule interfacial diffusion are reported. Colorless macroporous SMP membranes with deformed macropores can recover back to the "memorized" photonic crystal microstructures and the corresponding iridescent structural colors when triggered by diffused small molecules.
View Article and Find Full Text PDFSelf-Driven Janus Ga/Mg Micromotors for Reducing Deep Bacterial Infection in the Treatment of Periodontitis.
Adv Healthc Mater
December 2024
Hard Tissue Development and Regeneration Laboratory, Harbin Medical University, Harbin, 150086, China.
A self-propulsion Janus gallium (Ga)/magnesium (Mg) bimetallic micromotor is designed with favorable biocompatibility and antimicrobial properties as a therapeutic strategy for periodontitis. The Janus Ga/Mg micromotors are fabricated by microcontact printing technique to asymmetrically modify liquid metallic gallium onto magnesium microspheres. Hydrogen bubbles produced by the magnesium-water reaction can provide the driving performance of up to 31.
View Article and Find Full Text PDFCell confinement by micropatterning induces phenotypic changes in cancer-associated fibroblasts.
Acta Biomater
January 2025
Translational Tumor Engineering Laboratory, Department of Biomedical Engineering, National University of Singapore, Singapore 119276, Singapore; Cancer Science Institute, National University of Singapore, Singapore 117599, Singapore; The N.1 Institute for Health, National University of Singapore, Singapore 117456, Singapore. Electronic address:
Article Synopsis
- * The research explored how the shape and confinement of CAFs on specially designed surfaces influence their morphology and gene expression, finding that certain conditions led to changes toward more inflammatory and desmoplastic CAF types.
- * The findings suggest that the way CAFs are physically arranged affects their response to cancer therapies, suggesting that understanding CAF plasticity can help develop better treatment strategies.
Rapid Whole-Plate Cell and Tissue Micropatterning Using a Budget 3D Resin Printer.
ACS Omega
October 2024
Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, New Jersey 08544, United States.
The ability to precisely pattern cells and proteins is crucial in various scientific disciplines, including cell biology, bioengineering, and materials chemistry. Current techniques, such as microcontact stamping, 3D bioprinting, and direct photopatterning, have limitations in terms of cost, versatility, and throughput. In this Article, we present an accessible approach that combines the throughput of photomask systems with the versatility of programmable light patterning using a low-cost consumer LCD resin printer.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!