Int J Food Microbiol
Department of Food Science and Technology, College of Agricultural and Life Sciences, Chungnam National University, Daejeon 305-764, South Korea.
Published: August 2004
To isolate a carotenoid-hyperproducing yeast, Phaffia rhodozyma was treated by low-dose gamma irradiation below 10 kGy. Through repeated rounds of gamma irradiation and visual screening, a mutant 3A4-8 was isolated. It produced 3.3 mg of carotenoid per gram of yeast, 50% higher carotenoid content than that of the unirradiated strain. Glucose and peptone were the most suitable carbon and nitrogen sources for production of carotenoid based on the growth experiment of the mutant under various carbon and nitrogen sources. This result suggests that low-dose gamma irradiation could be used as a means of mutagenesis for isolation of a carotenoid-hyperproducing strain of P. rhodozyma because only the carotenoid-hyperproducing yeast survives gamma irradiation by scavenging oxygen radicals generated by radiolysis of water.
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http://dx.doi.org/10.1016/S0168-1605(03)00311-8 | DOI Listing |
Asian J Transfus Sci
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Department of Neurology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Nan Fang Yi Ke Da Xue Xue Bao
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School of Biomedical Engineering, Southern Medical University, Guangzhou 510515, China.
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Medical Technology, Health Information and Research Directorate, Ministry of Health, Jerusalem, Israel.
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