Arthrobacter aurescens TC1 atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli.

Appl Environ Microbiol

Department of Soil, Water, and Climate, Center for Microbial and Plant Genomics, 439 Borlaug Hall, 1991 Upper Buford Cir., University of Minnesota, St. Paul, MN 55108, USA.

Published: July 2004

Arthrobacter aurescens strain TC1 metabolizes atrazine to cyanuric acid via TrzN, AtzB, and AtzC. The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Escherichia coli. Hybridization studies localized trzN, atzB, and atzC to a 380-kb plasmid in A. aurescens strain TC1.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC444770PMC
http://dx.doi.org/10.1128/AEM.70.7.4402-4407.2004DOI Listing

Publication Analysis

Top Keywords

trzn atzb
20
atzb atzc
20
arthrobacter aurescens
8
atzc linked
8
functional escherichia
8
escherichia coli
8
aurescens strain
8
strain tc1
8
trzn
5
atzb
5

Similar Publications

Distribution of microbial taxa and genes degrading halogenated organic pollutants in the mangroves.

J Hazard Mater

January 2025

Department of Biology, College of Science, Shantou University, Shantou, Guangdong Province 515063, PR China. Electronic address:

Anthropogenic activities have led to serious contamination of halogenated organic pollutants (HOPs), such as PCBs, PBDEs, and HBCDs, in the mangrove wetland. Biodegradation of HOPs is generally driven by environmental microorganisms harboring dehalogenase genes. However, little is known if HOPs can affect the distributions of HOPs-degrading bacteria and dehalogenase genes in the mangrove wetlands.

View Article and Find Full Text PDF

A comparative evaluation of biochar and Paenarthrobacter sp. AT5 for reducing atrazine risks to soybeans and bacterial communities in black soil.

Environ Res

July 2024

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China; University of Chinese Academy of Sciences, Beijing, 100049, China; RWTH Aachen University, Institute for Environmental Research, WorringerWeg 1, 52074, Aachen, Germany. Electronic address:

Application of biochar and inoculation with specific microbial strains offer promising approaches for addressing atrazine contamination in agricultural soils. However, determining the optimal method necessitates a comprehensive understanding of their effects under similar conditions. This study aimed to evaluate the effectiveness of biochar and Paenarthrobacter sp.

View Article and Find Full Text PDF

Atrazine decontamination by a newly screened psychrotroph Paenarthrobacter sp. KN0901 in an aquatic system: Metabolic pathway, kinetics, and hydroponics experiment.

J Hazard Mater

September 2023

Key Laboratory of Wetland Ecology and Environment, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China. Electronic address:

Atrazine residues running off the fields and entering water resources are a major threat to food security and the ecosystem. In this study, a psychrotrophic functional strain named KN0901 to remove atrazine residues was screened. KN0901 could degrade 30 mg·L atrazine in 4 days at 15ºC with 10 CFU·mL incubation.

View Article and Find Full Text PDF

An atrazine-utilizing bacterium, designated as ZY, was isolated from agricultural soil and identified as . The ZY demonstrated a significant degradation capacity of atrazine, with the degradation efficiency of 12.5 mg L h in liquid media (at pH 7, 30°C, and the atrazine level of 100 mg L).

View Article and Find Full Text PDF

Microbes are crucial in removing various xenobiotics, including pesticides, from the environment, specifically by mineralizing these hazardous pollutants. However, the specific procedure of microbe-mediated pesticide degradation and its consequence on the environment remain elusive owing to limitations in culturing techniques. Therefore, in this study, we have investigated i) the physicochemical and elemental compositions of PCAS (pesticide-contaminated agricultural soils) and NS (natural soils); ii) the bacterial communities and degradation pathways, as well as some novel biodegradation genes (BDGs) and pesticide degradation genes (PDGs) across two different landscapes (PCAS and NS) by applying high-throughput sequencing.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!