Cation-pi interactions between aromatic amino acids and the positively charged residues lysine and arginine have been proposed to play an important role in stabilizing protein structure. We have used a peptide that adopts a coiled coil structure as a model system to evaluate the energetic contribution of cation-pi interactions to protein folding. Peptides were designed in which phenylalanine, tyrosine, and tryptophan were placed at a solvent-exposed position of the helix, one turn removed from an arginine residue that could provide a favorable cation-pi interaction. Only the arginine-phenylalanine pairing provided significant stabilization of the peptide structure and it appears that hydrophobic packing, rather than the cation-pi effect, is more likely to be responsible for the stability of this peptide. We conclude that any stabilizing effect of cation-pi interactions in these peptides is much smaller than that predicted from computational studies.
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http://dx.doi.org/10.1110/ps.04702104 | DOI Listing |
ACS Nano
January 2025
Department of Anesthesiology and Surgical Intensive Care Unit, Xinhua Hospital, School of Medicine and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China.
Identifying effective biomarkers has long been a persistent need for early diagnosis and targeted therapy of disease. While mass spectrometry-based label-free proteomics with trace cell has been demonstrated, deep proteomics with ultratrace human biofluid remains challenging due to low protein concentration, extremely limited patient sample volume, and substantial protein contact losses during preprocessing. Herein, we proposed and validated lanthanide metal-organic framework flowers (MOF-flowers), as effective materials, to trap and enrich protein in biofluid jointly through cation-π interaction and O-Ln coordination.
View Article and Find Full Text PDFRev Physiol Biochem Pharmacol
January 2025
Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK.
Since the first Chapter dealt with the well-known charge-charge interactions familiar to biologists, this concluding Chapter introduces some key electrical forces, probably much less familiar, perhaps even unknown. LLPS (liquid liquid phase separation) which we have seen involved in so much of cell biology depends on multivalent, π-π and cation-π electrical forces. How these arise is dealt with here and may be especially useful as an aide memoir to return to when such issues arise within the bulk of the text.
View Article and Find Full Text PDFJ Mol Model
January 2025
Department of Chemistry, Handique Girls' College, Guwahati , 781001, Assam, India.
Context: Cation-π and cation-lone pair interactions between 3d-metal (II) ions [Fe(II), Co(II), Ni(II) and Cu(II)] and furan are explored in the formation of 1:1 and 1:2 type complexes. Both cation-π (IE = -192.27 to -312.
View Article and Find Full Text PDFMol Divers
January 2025
Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, People's Republic of China.
Succinate dehydrogenase (SDH) has been identified as one of the ideal targets for the development of novel nematicides. However, the resistance of nematodes to fluopyram, one of the commercialized SDH inhibitors, is becoming a growing concern. Since expanding the structural diversity around an active scaffold is a useful strategy for drug development, herein a series of fluopyram analogues with a broad, biologically relevant indole moiety were synthesized and evaluated for nematicidal activity against C.
View Article and Find Full Text PDFACS Omega
January 2025
Department of Chemical Engineering, Konya Technical University, 42250 Konya, Türkiye.
This study investigated the adsorptive properties of functionalized fabric containing dimethylaminomethyl calix[4]arene (DMAM-Calix) to remove anionic methyl orange (MO) and cationic Rhodamine B (RhB) dyes in aqueous media. Adsorption studies were performed using a filtration system packed with DMAM-Calix-functionalized fabric (). The results revealed that the cationic and anionic structures work compatibly in a binary mixture medium.
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