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Mechanical positional information guides the self-organized development of a polygonal network of creases in the skin of mammalian noses.
Curr Biol
November 2024
Laboratory of Artificial & Natural Evolution (LANE), Department of Genetics & Evolution, University of Geneva, 1211 Geneva, Switzerland; SIB Swiss Institute of Bioinformatics, 1211 Geneva, Switzerland. Electronic address:
The glabrous skin of the rhinarium (naked nose) of many mammalian species exhibits a polygonal pattern of grooves that retain physiological fluid, thereby keeping their nose wet and, among other effects, facilitating the collection of chemosensory molecules. Here, we perform volumetric imaging of whole-mount rhinaria from sequences of embryonic and juvenile cows, dogs, and ferrets. We demonstrate that rhinarial polygonal domains are not placode-derived skin appendages but arise through a self-organized mechanical process consisting of the constrained growth and buckling of the epidermal basal layer, followed by the formation of sharp epidermal creases exactly facing an underlying network of stiff blood vessels.
View Article and Find Full Text PDFRainbow screening: Chromoproteins enable visualized molecular cloning.
Biotechnol J
April 2024
School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.
Article Synopsis
- Molecular cloning allows the combination of different DNA fragments into plasmids that replicate in host cells, typically requiring expensive and complex screening methods like blue-white screening to identify successful clones.
- The new Rainbow Screening method streamlines this process by using chromoproteins for easy visual identification of E. coli colonies, which reduces the need for extra genetic modifications and costs.
- This method has shown over 80% efficiency in assembling DNA fragments and accurately identifying correct constructs, potentially advancing molecular cloning techniques in biotechnology and synthetic biology.
Graphene Quantum Dots (GQD)-Mediated dsRNA Delivery for the Control of Fusarium Head Blight Disease in Wheat.
ACS Appl Bio Mater
March 2024
Department of Agronomy, Purdue University, West Lafayette, Indiana 47907, United States.
Graphene quantum dots (GQDs), a class of fluorescent carbon materials, have displayed significant potential in various fields such as energy devices, catalysis, sensing, bioimaging, and drug delivery. Because of their extremely small size, generally less than 100 nm, they also have tremendous potential in plant science research, especially for the delivery of nucleic acids, breaking the barrier of cell walls. In this study, we synthesized GQDs with a size range of 2-5 nm, characterized them, and surface-functionalized them with branched polyethylenimine (bPEI).
View Article and Find Full Text PDFGenomic Analysis Points to Multiple Genetic Mechanisms for Non-Transformable ST-50.
Microorganisms
February 2024
Department of Food, Nutrition and Bioprocessing Sciences, North Carolina State University, Raleigh, NC 27695, USA.
and are well known for their natural competence, i.e., their capacity for the uptake of naked DNA with subsequent transformation.
View Article and Find Full Text PDFTissue-targeted and localized AAV5-DCN and AAV5-PEDF combination gene therapy abrogates corneal fibrosis and concurrent neovascularization in rabbit eyes in vivo.
Ocul Surf
April 2024
Harry S. Truman Memorial Veterans' Hospital, Columbia, MO, 65201, USA; Mason Eye Institute, University of Missouri, Columbia, MO, 65212, USA.
Article Synopsis
- This study examines the use of adeno-associated virus5 (AAV5) to deliver the genes for decorin (DCN) and pigment epithelium-derived factor (PEDF) to treat corneal fibrosis and neovascularization (CNV) caused by chemical trauma in rabbits.
- Results showed that this gene therapy significantly decreased levels of corneal fibrosis and CNV compared to untreated eyes, with improved histological outcomes and increased induction of apoptotic cell death in neovessels.
- The therapy was found to be tolerable with no significant toxicity, suggesting potential for further research and application in treating ocular trauma-related vision impairments.
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