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Spontaneous Splenic Rupture Leading to the Diagnosis of CML.
J Community Hosp Intern Med Perspect
January 2025
Department of Internal Medicine, Temple University Health System, Philadelphia, PA, USA.
Background: Spontaneous splenic rupture (SSR) is a known complication of hematologic malignancy. Rare cases have been reported in which patients presented with SSR prior to diagnosis of chronic myeloid leukemia (CML). We present a case of atraumatic SSR due to CML presenting as persistent abdominal pain.
View Article and Find Full Text PDFWells' Syndrome Associated With Idiopathic Hypereosinophilic Syndrome in a Child: A Case Report.
Cureus
December 2024
Dermatology and Venereology, Faculty of Medicine, Al-Azhar University, Cairo, EGY.
Wells' syndrome is a rare inflammatory disease characterized by recurrent, erythematous plaques with histological flame figures, which can be associated with idiopathic hypereosinophilic syndrome (IHES). We present a case of a nine-year-old boy who presented with a one-year history of an itchy rash on his legs associated with peripheral eosinophilia. The rash initially started as an annular plaque and developed raised borders with central hyperpigmentation.
View Article and Find Full Text PDFA Novel Variant of GP9 Gene Resulting in Bernard-Soulier Syndrome: A Case Report.
Cureus
December 2024
Pediatric, Armed Forces Hospital Southern Region, Khamis Mushait, SAU.
Bernard-Soulier syndrome (BSS) is a rare autosomal recessive condition that is defined by low platelet count and platelet dysfunction characterized by the absence or dysfunction of the complex on the platelet surface. It is characterized by large defective platelets and thrombocytopenia. BSS is usually presented early in life.
View Article and Find Full Text PDFExtreme migratory connectivity and apparent mirroring of non-breeding grounds conditions in a severely declining breeding population of an Afro-Palearctic migratory bird.
Sci Rep
January 2025
British Trust for Ornithology, The Nunnery, Thetford, Norfolk, IP24 2PU, UK.
Understanding the distribution of breeding populations of migratory animals in the non-breeding period (migratory connectivity) is important for understanding their response to environmental change. High connectivity (low non-breeding population dispersion) may lower resilience to climate change and increase vulnerability to habitat loss within their range. Very high levels of connectivity are reportedly rare, but this conclusion may be limited by methodology.
View Article and Find Full Text PDFTheoretical and practical considerations for validating antigen-specific B cell ImmunoSpot assays.
J Immunol Methods
January 2025
Research & Development Department, Cellular Technology Limited, Shaker Heights, OH 44122, USA. Electronic address:
Owing to their ability to reliably detect even very rare antigen-specific B cells in cellular isolates such as peripheral blood mononuclear cells (PBMC), and doing so robustly in a high throughput-compatible manner, B cell ELISPOT/FluoroSpot (collectively "B cell ImmunoSpot") tests have become increasingly attractive for immune monitoring in regulated settings. Presently, there are no guidelines for the qualification and validation of B cell ImmunoSpot assay results. Here, we propose such guidelines, building on the experience acquired from T cell ImmunoSpot testing in an environment adhering to the requirements of regulatory bodies yet taking the unique features of B cell assays into account.
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