The results of identification of Mycobacterium non-tuberculosis isolated in the North-West Region of the Russian Federation, which have been obtained in the past 20 years, are presented. The identification was made by bacteriological and biochemical studies. M. avium-intracellulare complex is the man causative agent of mycobacteriosis. The currently available methods of chromatography and molecular genetics, which are designed for accelerated detection and species-specific identification of Mycobacterium, are briefly reviewed.
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Expert Rev Proteomics
January 2025
Biozentrum University of Basel, Basel, Switzerland.
Introduction: Recent work identified members of the evolutionarily conserved coronin protein family as key regulators of cell population size. This work originated ~25 years ago through the identification, by two-dimensional gel electrophoresis, of coronin 1 as a host protein involved in the virulence of Mycobacterium tuberculosis. We here describe the journey from a spot on a 2D gel to the recent realization that coronin proteins represent key controllers of eukaryotic cell population sizes, using ever more sophisticated proteomic techniques.
View Article and Find Full Text PDFInfect Dis Rep
January 2025
Department of Physiology, The "Carol Davila" University of Medicine and Pharmacy, 8 Eroii Sanitari Boulevard, 050474 Bucharest, Romania.
Spondylodiscitis can be caused by various microorganisms and has shown a continuous rise in incidence and mortality. The purpose of our study was to analyze the demographic and laboratory data, as well as comorbidities of patients that were surgically treated for spondylodiscitis in our hospital. The causative pathogens involved in the etiology of spinal infections were also assessed.
View Article and Find Full Text PDFFront Microbiol
January 2025
Department of Nephrology, Shengjing Hospital of China Medical University, Shenyang, China.
Introduction: The mortality rate associated with (MTB) has seen a significant rise in regions heavily affected by the disease over the past few decades. The traditional methods for diagnosing and differentiating tuberculosis (TB) remain thorny issues, particularly in areas with a high TB epidemic and inadequate resources. Processing numerous images can be time-consuming and tedious.
View Article and Find Full Text PDFHeliyon
January 2025
Clinical Laboratory Center, Hangzhou Red Cross Hospital, Hangzhou, Zhejiang, 310003, China.
Background: Diseases caused by (MTB) and non-tuberculous mycobacteria (NTM) have similar clinical symptoms but require different treatments. Rapid and accurate identification of MTB and NTM is essential for proper patient management and treatment.
Methods: To develop and assess a multiplex real-time fluorescence PCR (Multiplex PCR) method for rapid identification of MTB, complex (MAC), M.
Front Cell Infect Microbiol
January 2025
Department of Spine Surgery, Fuzhou Second General Hospital, Fuzhou, Fujian, China.
Background: This study aimed to evaluate the efficacy of metagenomic next-generation sequencing (mNGS) technology for identifying pathogens associated with spinal infection (SI).
Methods: A retrospective analysis was conducted on clinical data from 193 patients with suspected SI between August 2020 and September 2024. Based on histopathological results, the patients were divided into the SI group (n=162) and the non-SI group (n=31).
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