AI Article Synopsis
- The study investigates the role of T type Ca(2+) channels in the automaticity of cardiac precursor cells during embryonic development, focusing on how these channels change over time.
- Using patch clamp techniques, researchers found that while Ni(2+) effectively reduced spontaneous beating in early and intermediate stage cardiac cells, half of the late-stage cells remained unaffected by Ni(2+).
- The results suggest that the expression and impact of Ni(2+)-sensitive T-type Ca(2+) channels in Nkx2.5-positive cardiac precursor cells is regulated by the cells’ developmental stage.
Article Abstract
Background: It is controversial which subtypes of T type Ca(2+) channels are implicated in automaticity of cardiac cells during the embryonic period.
Method And Results: The effect of Ni(2+) on the automaticity of Nkx2.5-positive cardiac precursor cells sorted from embryonic stem cells during their differentiation was examined using patch clamp techniques. Although 40 micromol/L Ni(2+), which is enough to block Ni(2+)sensitive T type-Ca(2+) channels, decreased the spontaneous beating rate in all cells in the early and intermediate stage, Ni(2+) did not show any effects on the automaticity of 50% of the cells in the late stage.
Conclusion: These results indicate that Ni(2+)-sensitive T-type Ca(2+) channels expressed in the Nkx2.5-positive cardiac precursor cells are developmentally regulated.
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| http://dx.doi.org/10.1253/circj.68.724 | DOI Listing |
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