The detailed mechanism of eukaryotic 20S proteasome assembly is currently unknown. In the present study, we demonstrate that the 20S proteasome subunits alpha4 and alpha7 interact with each other as well as all the alpha-subunits in vivo and in vitro. The N-terminal parts of alpha4 and alpha7 are essential for these newly discovered interactions in vitro. Glycerol gradient centrifugation of soluble extracts of HEK293 cells and Western blot analyses show that several alpha-subunits are found in non-proteasomal low-density fractions. The alpha4 and alpha7 subunits co-immunoprecipitate together from these low-density fractions. The unexpected interaction between alpha4 and alpha7 may provide a molecular basis for the formation of previously reported 13S and 16S assembly intermediates.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.febslet.2004.05.067 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prenatal Hypoxia Predisposes to Impaired Expression of the and Genes in Adult Rats without Affecting Acetylcholine Metabolism during Embryonic Development.
Biochemistry (Mosc)
November 2024
Laboratory of Regulation of Brain Neuronal Functions, Pavlov Institute of Physiology, Russian Academy of Sciences, Saint Petersburg, 199034, Russia.
Previous studies have shown that the combined effect of fetal hypoxia and maternal stress hormones predetermines tendency to nicotine addiction in adulthood. This study in rats aimed to investigate the effect of prenatal severe hypoxia (PSH) on acetylcholine metabolism in the developing brain, as well as on expression of acetylcholine receptors and in both the developing brain and adult brain structures following nicotine consumption. In the developing brain of PSH rats, no changes were found in the activity of choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) or disturbances in the acetylcholine levels.
View Article and Find Full Text PDFWater-Soluble Lynx1 Upregulates Dendritic Spine Density and Stimulates Astrocytic Network and Signaling.
Mol Neurobiol
November 2024
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Secreted and membrane-tethered mammalian neuromodulators from the Ly6/uPAR family are involved in regulation of many physiological processes. Some of them are expressed in the CNS in the neurons of different brain regions and target neuronal membrane receptors. Thus, Lynx1 potentiates nicotinic acetylcholine receptors (nAChRs) in the brain, while others like Lypd6 and Lypd6b suppress it.
View Article and Find Full Text PDFArea-wide survey and monitoring of insecticide resistance in the brown planthopper, Nilaparvata lugens (Stål), from 2020 to 2023 in China.
Pestic Biochem Physiol
November 2024
College of Plant Protection, Nanjing Agricultural University / State & Local Joint Engineering Research Center of Green Pesticide-Invention and Application, Nanjing 210095, Jiangsu, China. Electronic address:
The brown planthopper (BPH), Nilaparvata lugens (Stål), is a notorious pest affecting Asian rice crops. The evolution of insecticide resistance in BPH has emerged as a significant challenge in effectively managing this pest. This study revealed the resistance status of BPH to nine insecticides in ten provinces and Shanghai City in China from 2020 to 2023.
View Article and Find Full Text PDFAn endogenous cholinergic system controls electrical conduction in the heart.
Eur Heart J
October 2024
State Key Laboratory of Cardiovascular Diseases, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China.
Article Synopsis
- - The study investigates the presence and role of an intrinsic cholinergic system in the heart, which uses acetylcholine (ACh) to manage electrical conduction and its link to arrhythmias in humans and mice.
- - Researchers identified key components of this system, such as ACh receptors and transporters, showing that they significantly influence how electrical signals are conducted in heart cells.
- - Findings suggest that defects in this cholinergic system can lead to serious ventricular arrhythmias, highlighting new mechanisms for understanding heart rhythm disorders beyond traditional views.
Neuronal nicotinic acetylcholine receptor antibodies in autoimmune central nervous system disorders.
Front Immunol
June 2024
Second Department of Neurology, School of Medicine, "Attikon" University Hospital, National and Kapodistrian University of Athens, Athens, Greece.
Background: Neuronal nicotinic acetylcholine receptors (nAChRs) are abundant in the central nervous system (CNS), playing critical roles in brain function. Antigenicity of nAChRs has been well demonstrated with antibodies to ganglionic AChR subtypes (i.e.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!