AI Article Synopsis
- Delta-lactoferrin (deltaLf) mRNA, derived from alternative splicing of the Lf gene, is typically absent in malignant tissues but present in normal ones, indicating its potential as a prognostic marker in breast cancer.
- Recent studies involved producing the deltaLf protein, which was confirmed to be a 73 kDa protein that functions primarily in the cytoplasm.
- The expression of deltaLf in cells leads to cell cycle arrest and inhibits cell proliferation, suggesting it may be crucial for regulating normal cell growth.
Article Abstract
Delta-lactoferrin (deltaLf) mRNA is the product of alternative splicing of the Lf gene. It has been found in normal tissues and was reported to be absent from their malignant counterparts. Our recent investigations have shown that deltaLf expression is a good prognostic indicator in human breast cancer. However, deltaLf has up till now only been identified as a transcript, and in order to characterize the deltaLf protein and determine its function we have used a deltaLf cDNA construct to produce the protein in vitro and in vivo. A 73 kDa protein was immunoprecipitated from in vitro translation products and this molecular weight is in accordance with the use of the first in frame AUG start codon located in exon 2. We also produced a cell line expressing deltaLf under doxycycline induction. Using this model we have been able to show that deltaLf is mainly distributed in the cytoplasm. Its expression induces cell cycle arrest and inhibits cell proliferation. Our results suggest that deltaLf may play an important role in the regulation of normal cell growth.
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| http://dx.doi.org/10.1023/b:biom.0000027712.81056.13 | DOI Listing |
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