AI Article Synopsis
- Three pumpkin embryogenic lines were developed from wounded zygotic embryos, influenced by the presence of 2,4-D and nitrogen sources in the culture medium.
- Early embryo stages showed the highest levels of DNA methylation, particularly in conditions with 2,4-D and certain nitrogen sources, linking methylation to early development.
- As embryos matured, DNA methylation levels decreased, but the embryogenic traits of the pumpkin tissue remained intact after exposure to a methylation inhibitor, 5-azaC, for two months.
Article Abstract
Three pumpkin embryogenic lines were initiated on wounded zygotic embryos cultured on medium with or without 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryo development was controlled by the availability of various compounds in the medium: presence/absence of 2,4-D, nitrogen sources. The highest rate of DNA methylation was in the early embryo stages, predominantly on MSC medium with 2,4-D and on auxin-free medium supplemented with 1.0 m M NH(4)Cl. DNA methylation was correlated with early embryo development in a manner that was not exclusively dependent on the presence/absence of exogenous auxin. DNA methylation decreased during embryo maturation on auxin-free MSC medium and on auxin-free MSC supplemented with 12.3 micro M 5-azacytidine (5-azaC). The embryogenic features of the pumpkin tissue were preserved, even after a 2-month treatment with 5-azaC.
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| http://dx.doi.org/10.1007/s00299-004-0819-6 | DOI Listing |
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