AI Article Synopsis
- The study examined the response of monocytes to bacterial lipopolysaccharide (LPS) in patients with aggressive periodontitis compared to those without.
- By using whole-blood cultures and analyzing cell activation markers, researchers found that LPS stimulation led to similar immune responses in both groups.
- Ultimately, the findings suggest that there was no hyper-responsiveness of monocytes to P. gingivalis LPS in the aggressive periodontitis patients examined.
Article Abstract
In this study, we re-visited the issue of hyper-responsiveness of monocytes to bacterial lipopolysaccharide (LPS) in aggressive periodontitis patients. We used whole-blood cultures to compare monocyte activation by Porphyromonas gingivalis LPS between Thai subjects with generalized aggressive periodontitis and those without periodontitis. Upon stimulation with P. gingivalis LPS, expression of co-stimulatory molecules on monocytes and expression of CD69 on NK and gamma delta T-cells were analyzed by flow cytometry, and the production of interleukin-1 beta and prostaglandin E(2) was monitored by ELISA. LPS stimulation resulted in a dose-dependent up-regulation of CD40, CD80, and CD86 on monocytes, and up-regulation of CD69 on NK cells and gamma delta T-cells in both the periodontitis and non-periodontitis groups. The levels of activation markers and the mediator production after LPS stimulation were quite similar for both groups. In conclusion, we did not observe hyper-responsiveness of monocytes to P. gingivalis LPS challenge in Thai patients with aggressive periodontitis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1177/154405910408300706 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fabrication of Mastic Gum Resin Tethered Phospholipid Nanocarriers for the Evaluation and Enhancement of Anti-inflammatory and Anti-bacterial Effects.
Curr Pharm Des
January 2025
Department of Pharmaceutics, PSG College of Pharmacy, Coimbatore 600 004, Tamil Nadu, India.
Introduction: The objective of the present study was to improve the anti-inflammatory and antibacterial activities of mastic gum resin (MGR). MGR was loaded into a phospholipid nanocarrier with or without partially hydrolyzed ginsenoside, followed by dispersion into distilled water.
Method: The phospholipid nanocarrier dispersion showed significantly enhanced in-vitro release, porcine skin/ intestine permeation, and retention.
Nucleotide-binding oligomerization domain-like receptor family caspase recruitment domain containing protein 5 affects the progression of periodontitis by regulating the function of periodontal membrane cells.
J Dent Sci
January 2025
Department of Periodontology, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China.
Background/purpose: Nucleotide-binding oligomerization domain-like receptor family caspase recruitment domain containing protein 5 (NLRC5) plays a regulatory role in innate and adaptive immunity. However, its role in periodontitis remains unclear. This study investigated the effects of NLRC5 on periodontitis and the underlying mechanism.
View Article and Find Full Text PDFCeO@CA-074Me Nanoparticles Alleviate Inflammation and Improve Osteogenic Microenvironment by Regulating the CTSB-NLRP3 Signaling Pathway.
Int J Nanomedicine
January 2025
Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, People's Republic of China.
Background: It is well established that the interaction between osteogenesis and inflammation can impact bone tissue regeneration. The use of nanoparticles to treat and alleviate inflammation at the molecular level has the potential to improve the osteogenic microenvironment and serve as a therapeutic approach.
Methods: We have synthesized new hollow cerium oxide nanoparticles and doped with cathepsin B inhibitor (CA-074Me) to create novel CeO@CA-074Me NPs.
Dipyridamole Attenuates Experimental Periodontitis by Regulating M1 Macrophage Polarization via PKA/PKG Pathways.
J Periodontal Res
January 2025
Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, China.
Aim: Periodontitis is a chronic inflammatory disease initiated by dysbiosis of the local microbial community. As a non-specific phosphodiesterase inhibitor, dipyridamole features anti-oxidant and anti-inflammatory properties. This study aimed to investigate the effects of dipyridamole in an experimental rat model of periodontitis.
View Article and Find Full Text PDFDynamin-Related Protein 1 Orchestrates Inflammatory Responses in Periodontal Macrophages via Interaction With Hexokinase 1.
J Clin Periodontol
January 2025
Department of Oral Implantology, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, China.
Aim: To explore the potential roles of mitochondrial dysfunction in the initiation of inflammation in periodontal macrophages and to determine the mechanism underlying the involvement of dynamin-related protein 1 (Drp1) in macrophage inflammatory responses through its interaction with hexokinase 1 (HK1).
Materials And Methods: Gingival tissues were collected from patients diagnosed with periodontitis or from healthy volunteers. Drp1 tetramer formation and phosphorylation were analysed using western blot.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!