AI Article Synopsis
- BPA (bisphenol A) is commonly found in consumer products like food can linings and baby bottles but is linked to reproductive toxicity in animals.
- A study examined how BPA affects protein expression in TM4 cells, crucial for sperm production, showing that higher BPA concentrations decreased cell viability significantly.
- Using two-dimensional electrophoresis (2-DE) and mass spectrometry, 11 proteins with altered expression due to BPA were identified, including HSP 27 and placental calcium binding protein.
Article Abstract
BPA, bisphenol A, a monomer of epoxy resins and polycarbonate plastic, is used in many consumer products including the plastic linings of cans for food and babies' bottles. BPA has been reported to cause reproductive toxicity and affects cells in rats and mice at high doses. In this study, the effect of BPA on protein expression in TM4 cells (a mouse Sertoli cell line) known to play an essential role in Spermatogenesis was investigated by two-dimensional electrophoresis (2-DE). After 16 h exposure to 50, 100, 150, 200, and 250 microM of BPA, the viability of TM4 cells decreased to about 90, 85, 78, 55, and 30% of control respectively. Approximately 800 protein spots in TM4 cells were analyzed by 2-DE with pH 4-7 linear immobilized pH gradient (IPG) Dry Strip, and 11 proteins which showed significantly different expression levels were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Among these, HSP 27 and placental calcium binding protein may be proteins differentially expressed by BPA exposure.
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| http://dx.doi.org/10.1271/bbb.68.1201 | DOI Listing |
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