Divergent roles for estrogens and androgens in the expression of female goat sexual behavior.

We tested the hypothesis that the activation of the androgen receptor (AR) is required for full expression of female goat sexual behavior. Once a week for 6 weeks, ovariectomized (OVX) females were given priming doses of progesterone 72 and 48 h before behavioral observation. Estradiol (E(2); 100 microg), testosterone (T; 100 mg), or sesame oil was supplied 14 h before behavioral testing. Six goats received the AR antagonist flutamide (9 mg/kg sc) 8 h before and 4 h after steroid injection. Six goats received the carrier only. After 3 weeks, flutamide and carrier treatments were switched so that all females received all treatments. Treatments with E(2) and T were equally effective in eliciting estrus-typical behaviors (sniffing, courting, leg kicks, mount attempts by males, bouts of thrusting by males, ejaculations, and flehman responses) compared to treatment with oil. Flutamide treatment enhanced proceptive behaviors in E(2)-treated females compared to other treatment groups; this was most likely via enhanced tail wagging. Moreover, compared to goats given T + carrier, T + flutamide significantly reduced receptivity in females. The results of this experiment implicate the AR as an important facilitator of some aspects of the female goat sexual behavior. However, the results of this experiment do not show whether androgens influence estrous behaviors alone or in some combination with estrogen.