Background: The possibility of detection of lymphocytes T specific sensitivity after contact with the allergen (mainly food allergen) even in early age of children has been reported in this paper.
Methods/data Base: This is the literature review of basic applied methods such as: lymphocyte blastic transformation in allergen stimulated culture, macrophage migration inhibition test and flow cytometric analysis of in vivo and in vitro lymphocyte specific activation by using activation markers (CD69, PCNA - proliferating cell nuclear antigen and others).
Results/conclusions: The significance of such investigation is underlined especially for young children during early diagnosis of food allergy and monitoring of the treatment.
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