Purpose: To examine the effect of trypan blue staining on the biomechanical behavior of the porcine anterior lens capsule.
Setting: Department of Ophthalmology, Technical University of Dresden, Dresden, Germany.
Methods: Fifty-five anterior lens capsules from porcine cadaver eyes were used. Two parallel 8.0 mm x 4.0 mm large capsule strips were prepared from each capsule. After trypan blue staining for various time intervals combined with exposure to white light (6000 lux) or with no light exposure, biomechanical stress-strain measurements were performed using an automated material tester. Untreated specimens and specimens treated with glutaraldehyde 0.1% were used as controls. The absorption spectrum of trypan blue 0.1% solution and the emission spectrum of the light source were measured.
Results: After treatment with light and trypan blue, at 25% strain, there was a statistically significant increase in stress of up to 70.1% and in elastic stiffness of 47% and a decrease in the ultimate mechanical strain of up to 13%. There were no biomechanical changes in capsules with trypan blue staining in the absence of light or after a short illumination time of 30 seconds, indicating a light-dependent process. After 30 minutes of glutaraldehyde 0.1% treatment, there was an increase in stress of 321.6% at 25% strain and a decrease in the ultimate strain of 47.6%. The emission spectrum of the light source included the absorption peak for trypan blue at 580 nm.
Conclusions: Trypan blue staining of the lens capsule combined with light irradiation for at least 1 minute led to an increase in elastic stiffness at 25% strain and a reduction in the ultimate extensibility. This effect is probably due to the photosensitizing action of trypan blue, leading to light-induced collagen crosslinking of the capsule collagen similar to age-related crosslinking. Nucleus expression might be impeded by the increased capsule stiffness. Continuous curvilinear capsulorhexis is facilitated.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jcrs.2003.11.031 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Common salt (NaCl) causes developmental, behavioral, and physiological defects in .
Nutr Neurosci
January 2025
Neural Developmental Biology Lab, Department of Life Science, NIT Rourkela, Rourkela, Odisha, India.
Purpose: The incidence of obesity has surged to pandemic levels in recent decades. Approximately 1.89 million obesity are linked to excessive salt consumption.
View Article and Find Full Text PDFInvestigating the effect of femtosecond laser pulses on noscapine and curcumin cellular uptake and improving drug delivery in gastric cancer cells.
Heliyon
January 2025
Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran.
The MKN45 cell line, a type of gastric cancer cell, exhibits resistance to chemotherapy agents through various mechanisms. Curcumin and noscapine, two plant-derived anticancer compounds, exhibit selective cytotoxicity towards cancer cells. However, their bioavailability is poor both in vitro and in vivo.
View Article and Find Full Text PDFSerum starvation as the most suitable method for inducing G/G phase cell cycle arrest in six-banded armadillo (Euphractus sexcinctus) skin-derived fibroblast lines.
Tissue Cell
December 2024
Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid (UFERSA), Mossoró, RN, Brazil. Electronic address:
Background: Several studies have evaluated different cell cycle synchronization methods to improve reprogramming efficiency aimed at wild species conservation. The six-banded armadillo is one of the wild mammals with significant ecological and biomedical interests but has not yet been evaluated for reprogramming purposes.
Objective: We investigated the effects in a time-dependent manner of serum starvation (SS; 0.
Citrate-modified bacterial cellulose as a potential scaffolding material for bone tissue regeneration.
PLoS One
December 2024
Faculty of Science, Department of Biosciences, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia.
Bacterial cellulose (BC) is a novel biocompatible polymeric biomaterial with a wide range of biomedical uses, like tissue engineering (TE) scaffolds, wound dressings, and drug delivery. Although BC lacks good cell adhesion due to limited functionality, its tunable surface chemistry still holds promise. Here, hydroxyapatite (HA) was incorporated into a citrate-modified BC (MBC) using the biomimetic synthesis in simulated body fluid (SBF).
View Article and Find Full Text PDFDevelopment of a novel and robust CuO-CoO@Biochar nanocomposite using Terminalia chebula leaf extract for reduction of nitro compounds and photodegradation of single and binary mixture of organic contaminants.
Environ Sci Pollut Res Int
December 2024
Department of Chemistry, National Institute of Technology, Silchar, 788010, Assam, India.
In this work, Terminalia chebula leaf extract was used to synthesize CuO-CoO nanoparticles, which were then embedded in a rice straw biochar. This new biochar-based nano-catalyst is used to photocatalytically degrade a variety of dyes (Eosin Y, Trypan Blue, Crystal Violet, Methylene Blue, Brilliant Green), as well as a binary mixture of Eosin Y and Trypan Blue dyes. It is also used for the catalytic reduction of nitro compounds (4-NP, 3-NP, and Picric acid).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!