Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave.

Opt Lett

Department of Electronics and Electrical Engineering, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.

Published: June 2004

Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (approximately 43 degrees C) was effective in improving the transfection efficiency.

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http://dx.doi.org/10.1364/ol.29.001227DOI Listing

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