Three mutants with Tn5-B21 insertion in tonB3 (PA0406) of Pseudomonas aeruginosa exhibited defective twitching motility and reduced assembly of extracellular pili. These defects could be complemented with wild-type tonB3.
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http://dx.doi.org/10.1128/JB.186.13.4387-4389.2004 | DOI Listing |
Int J Microbiol
December 2024
Department of Microbiology, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
PLoS Pathog
December 2024
Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, United Kingdom.
Type IV pili (T4Ps) are abundant in many bacterial and archaeal species, where they play important roles in both surface sensing and twitching motility, with implications for adhesion, biofilm formation and pathogenicity. While Type IV pilus (T4P) structures from other organisms have been previously solved, a high-resolution structure of the native, fully assembled T4P of Pseudomonas aeruginosa, a major human pathogen, would be valuable in a drug discovery context. Here, we report a 3.
View Article and Find Full Text PDFACS Appl Mater Interfaces
December 2024
School of Polymer Science and Polymer Engineering, The University of Akron, Akron, Ohio 44325, United States.
Modulating microbial motility and physiology can enhance the production of bacterial macromolecules and small molecules. Herein, a platform of water-soluble and amphiphilic peptidomimetic polyurethanes is reported as a means of regulating bacterial surface behavior and the concomitant production of extracellular polymeric substances (EPS). It is demonstrated that carboxyl (-COOH)-containing polyurethanes exhibited 17-fold and 80-fold enhancements in () swarming and twitching areas, respectively.
View Article and Find Full Text PDFMicrobiol Spectr
December 2024
Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
bioRxiv
November 2024
Department of Microbiology, ADA Forsyth Institute, Cambridge MA, 02142, USA.
All cultivated Patescibacteria, or CPR, exist as obligate episymbionts on other microbes. Despite being ubiquitous in mammals and environmentally, molecular mechanisms of host identification and binding amongst ultrasmall bacterial episymbionts are largely unknown. Type 4 pili (T4P) are well conserved in this group and predicted to facilitate symbiotic interactions.
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