Learning and memory are defective in the Drosophila mutant rutabaga, which has a low intracellular cyclic adenosine monophosphate (cAMP) concentration. The aim of this study was to compare modulation effects of protein kinase C activator (PKC-A) on the delayed-rectifier potassium current (IKDR) in wild-type and rutabaga neurons. IKDR was measured from cultured (2 days) wild-type and rutabaga neurons. The authors examined the effects of PKC-A on IKDR in wild-type and rutabaga neurons. IKDR was measured from neurons before and after addition of PKC-A to the external solution. IKDR was smaller in rutabaga neurons (380 +/- 25 pA) than in wild-type neurons (529 +/- 44 pA). IKDR was reduced by PKC-A more in wild-type (decreasing 55 +/- 6%) than in rutabaga (decreasing 35 +/- 8%) neurons (single-cell studies). In the presence of PKC-A, there was no difference in IKDR between wild-type (229 +/- 31 pA) and rutabaga (242 +/- 26 pA) neurons (population studies). These results indicate that PKC-A differentially affects the delayed-rectifier channel in wild-type rutabaga.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/00207450490430480 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The glutathione S-transferase BnGSTU12 enhances the resistance of Brassica napus to Sclerotinia sclerotiorum through reactive oxygen species homeostasis and jasmonic acid signaling.
Plant Physiol Biochem
December 2024
Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City and Southwest University, College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, 400715, China; Academy of Agricultural Sciences, Southwest University, Beibei, Chongqing, 400715, China; Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, 400715, China. Electronic address:
Sclerotinia sclerotiorum is a severe disease that affects rapeseed (Brassica napus), resulting in significant yield losses. In previous study, we identified the candidate GLUTATHIONE S-TRANSFERASE (GST) gene, BnGSTU12, associated with sclerotiorum stem resistance and the expression levels of BnGSTU12 in resistant lines were higher than that in susceptible lines. We analyzed the function of the BnGSTU12 during S.
View Article and Find Full Text PDFBnaHSFA2, a heat shock transcription factor interacting with HSP70 and MPK11, enhances freezing tolerance in transgenic rapeseed.
Plant Physiol Biochem
December 2024
State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China. Electronic address:
Heat shock transcription factors (Hsfs) play important roles in plant developmental regulations and various abiotic stress responses. However, their evolutionary mechanism of freezing tolerance remains poorly understood. In our previous transcriptomics study based on DNA methylation sequencing, the BnaHsfA2 was found to be significantly accumulated in winter rapeseed (Brassica rapa L.
View Article and Find Full Text PDFThe role of sugar transporter BrSWEET11 in promoting plant early flowering and preliminary exploration of its molecular mechanism.
Plant Cell Rep
December 2024
School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, 453003, China.
BrSWEET11 accelerated Arabidopsis thaliana flowering, while silencing Brsweet11 in Brassica rapa delayed flowering relative to controls. BrSWEET11 is involved in sucrose transport after being induced by long-day conditions. SWEETs (Sugars Will Eventually Be Exported Transporters) are sugar outflow transporters that may participate in the regulation of plant flowering.
View Article and Find Full Text PDFA visible seedling-stage screening system for the Brassica napus hybrid breeding by a novel hypocotyl length-regulated gene BnHL.
Plant Biotechnol J
November 2024
Key Laboratory for Bio-Resources and Eco-Environment, College of Life Sciences, Sichuan University, Chengdu, China.
Article Synopsis
- Rapeseed (Brassica napus) is a vital oilseed crop that benefits from heterosis, but using recessive genic male sterility (RGMS) for breeding can be slow and labor-intensive due to the need to remove fertile plants.
- Researchers identified the BnHL gene, linked to a fertility gene (BnMs2), as a potential morphological marker for identifying fertile plants based on hypocotyl length, which showed that edited mutants had longer hypocotyls compared to wild types.
- The study demonstrated that by targeting the BnHL gene, breeders can efficiently distinguish fertile plants early in the process, enhancing RGMS breeding methods without introducing transgenes throughout production.
Zinc finger transcription factors BnaSTOP2s regulate sulfur metabolism and confer Sclerotinia sclerotiorum resistance in Brassica napus.
J Integr Plant Biol
November 2024
National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Rapeseed (Brassica napus L.) exhibits high-sulfur requirements to achieve optimal growth, development, and pathogen resistance. Despite the importance of sulfur, the mechanisms regulating its metabolism and disease resistance are not fully understood.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!