Background: Thymus and activation regulated chemokine (TARC) is a CC chemokine that attracts CCR4+ T cells. We reported previously that TARC is an important chemokine that defines Th2 imbalance in the pathogenesis of atopic dermatitis (AD).
Objectives: This study was undertaken to clarify TARC producing cells in peripheral blood mononuclear cells (PBMCs), the regulation of dust mite-allergen clude extract (DME) and different immunosuppressive drugs (Tacrolimus (FK506), cyclosporine (CsA), dexamethasone (Dex)) on TARC production by peripheral PBMCs from AD patients in vitro.
Methods: Monocyte derived dendritic cells (MoDCs) were generated from and TARC mRNA levels were examined and comapared with those from T cells in PBMCs from AD patients. PBMCs were cultured with or without DME and/or immunosuppressive drugs (Tacrolimus, CsA, Dex) for 7 days and TARC levels were measured.
Results: PBMCs from AD patients which were cultured with DME stimulation for 7 days showed significantly higher levels of TARC production than those from healthy controls. RT-PCR demonstrated that TARC mRNA was expressed in CD4+ T cells, CD8+ T cells and MoDCs. Tacrolimus, CsA and Dex individually suppressed TARC production by PBMCs from AD patients which were co-cultured with DME for 7 days. Gel shift analysis revealed differential inhibitory effects of these immunosuppressive drugs on NFkappaB activity in PBMCs from AD patients.
Conclusion: Our data demonstrate that TARC producing cells are MoDCs, T cells as well as epidermal keratinocytes in AD. We suggest that MoDCs might regulate the immune responses by attracting T cells and CD25+ T cells in the pathogenesis of AD. We also showed the important role of DME on TARC production and the inhibitory effect of the immunosuppressive drugs on TARC production by PBMCs from AD patients, that can regulate ongoing immune responses in the pathogenesis of AD.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jdermsci.2004.02.008 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Topical Anti-Inflammatory Effects of Quercetin Glycosides on Atopic Dermatitis-Like Lesions: Influence of the Glycone Type on Efficacy and Skin Absorption.
Inflammation
January 2025
Research Center for Food and Cosmetic Safety and Center for Drug Research and Development, Chang Gung University of Science and Technology, Kweishan, Taoyuan, Taiwan.
Atopic dermatitis (AD) is a multifaceted inflammatory skin condition characterized by the involvement of various cell types, such as keratinocytes, macrophages, neutrophils, and mast cells. Research indicates that flavonoids possess anti-inflammatory properties that may be beneficial in the management of AD. However, the investigation of the glycoside forms for anti-AD therapy is limited.
View Article and Find Full Text PDFHigh isolated 8-port MIMO antenna and 16-port massive antenna for mm wave (5G NR-n260) applications in time division duplex mode.
Sci Rep
December 2024
Department of Electronics & Communication, University of Allahabad, Prayagraj, UP, India.
In this article an 8-port annular ring-shaped MIMO antenna for 5G and 5G advanced applications is presented. An annular ring on the radiating plane and novel isolator structure on the ground plane are etched over a Rogers RT/Duorid (5870 tm) substrate to achieve high performance antenna for mm wave applications. A systematic study is performed, and an optimized single port antenna (Design-4) is selected among Designs (1-4).
View Article and Find Full Text PDFExtracellular ATP Contributes to Barrier Function and Inflammation in Atopic Dermatitis: Potential for Topical Treatment of Atopic Dermatitis by Targeting Extracellular ATP.
Int J Mol Sci
November 2024
Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Article Synopsis
- - Atopic dermatitis (AD) involves chronic inflammation and a weakened skin barrier, worsened by factors like scratching, and this study looks into how extracellular ATP affects AD.
- - The research found that ATP reduces filaggrin, a protein vital for skin barrier strength, and heightens inflammation by stimulating immune cell activity when released due to IL-4 and mechanical triggers.
- - Clodronate, which inhibits ATP release, shows promise in restoring filaggrin levels and reducing inflammation in a mouse model of AD, suggesting that targeting ATP could be a new way to treat this condition.
Protective effect of Curcuma longa L. leaves and pseudostems extract against 1-chloro-2,4-dinitrobenzene-induced atopic dermatitis in BALB/c mice.
J Ethnopharmacol
February 2025
Department of Food Technology and Nutrition, Chonnam National University, Yeosu, 59626, Republic of Korea; Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu, 59626, Republic of Korea. Electronic address:
Ethnopharmacological Relevance: The perennial herbaceous plant, Curcuma longa L. (turmeric) is primarily grown and harvested for pharmacological purposes in China, Korea, and various tropical regions in South Asia. Turmeric has been used for centuries as an indigenous medicine.
View Article and Find Full Text PDFAnti-inflammatory Capacity of a Medicinal herb extract, , on and models-induced atopic dermatitis.
Heliyon
October 2024
Department of Integrated Drug Development and Natural Products, Graduate School, Kyung Hee University, Seoul, 02447, Republic of Korea.
Article Synopsis
- Bunge (AA), a plant from the Liliaceae family, has traditional uses for managing inflammation, but its effects on atopic dermatitis (AD) were previously unexplored.
- The study showed that topical AA ointment significantly reduced AD symptoms in mice and keratinocytes by improving skin conditions and reducing inflammation markers.
- AA works by lowering levels of certain cytokines and signaling molecules involved in skin inflammation, suggesting it could be a promising treatment for atopic dermatitis.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!