AI Article Synopsis
- Researchers developed degenerate primers based on known CYP4 sequences to amplify cDNA from frog liver mRNA, resulting in the identification of five new CYP genes.
- Three of the new genes were classified into the CYP4T subfamily, while two others were similar to CYP4F and CYP4V subfamilies.
- Two full-length cDNAs, CYP4F42 and CYP4V4, were characterized, with CYP4F42 expressed mainly in specific organs and CYP4V4 showing widespread expression.
Article Abstract
In order to obtain cDNA clones coding for CYP4 proteins in frog Xenopus laevis, degenerate primers were designed utilizing the conserved sequences of known CYP4s and were used to amplify partial cDNA fragments from liver mRNA. Five new CYP genes were identified. Three of these genes, XL-1, -2 and -3, were assigned to the CYP4T subfamily found previously in fish and amphibians. The other two genes, XL-4 and XL-5, were quite similar to CYP4F and CYP4V subfamilies, respectively. Subsequently, two full-length cDNA clones corresponding to XL-4 and XL-5 were isolated and characterized. The resultant cDNAs, designated as CYP4F42 and CYP4V4, had open reading frames encoding proteins of 528 and 520 residues, respectively. RT-PCR analysis indicated that the expression of CYP4F42 was limited to the liver, kidney, intestine and brain. In contrast, CYP4V4 mRNA was expressed ubiquitously.
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| http://dx.doi.org/10.1016/j.cbpc.2004.02.014 | DOI Listing |
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