To determine the rates of Enterobacter sakazakii (ES) infections among very low birth weight infants, culture data from the National Institute of Child Health and Human Development Neonatal Research Network were reviewed. Only one case of ES sepsis was identified among 10660 neonates. These data suggest that outside of the epidemic situation, ES is very rare in very low birth weight infants.
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http://dx.doi.org/10.1016/j.jpeds.2004.02.045 | DOI Listing |
Appl Environ Microbiol
January 2025
McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas, USA.
Electroactive organisms contribute to metal cycling, pollutant removal, and other redox-driven environmental processes via extracellular electron transfer (EET). Unfortunately, developing genotype-phenotype relationships for electroactive organisms is challenging because EET is necessarily removed from the cell of origin. Microdroplet emulsions, which encapsulate individual cells in aqueous droplets, have been used to study a variety of extracellular phenotypes but have not been applied to investigate EET.
View Article and Find Full Text PDFMicroorganisms
November 2024
State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
, an opportunistic foodborne pathogen, has a strong resistance to osmotic stress and desiccation stress, but the current studies cannot elucidate this resistance mechanism absolutely. A mechanosensitive channel MscM was suspected of involving to desiccation resistance mechanism of To investigate the specific molecular mechanism, the mutant strain (Δ) was constructed using the homologous recombination method, and the complementary strain was obtained by gene complementation, followed by the analysis of the difference between the wild-type (WT), mutant, and complementary strains. Compared to the wild-type bacteria (WT), the inactivation rate of the Δ strain decreased by 15.
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November 2024
Microbial Methods Development Branch, Division of Food and Environmental Safety, Office of Applied Microbiology and Technology (OAMT), Office of Laboratory Operations and Applied Science (OLOAS), Human Foods Program, Food & Drug Administration, College Park, MD 20740, USA.
Precision metagenomic approaches using Oxford Nanopore Technology (ONT) sequencing has been shown to allow recovery of complete genomes of foodborne bacteria from overnight enrichments of agricultural waters. This study tests the applicability of a similar approach for genome recovery from powdered infant formula (PIF) overnight enrichments, where typically dominates the overall microbiome (>90%). This study aimed to test whether using ONT sequencing of overnight PIF enrichments could recover a completely closed genome for further genomic characterization.
View Article and Find Full Text PDFPathogens
November 2024
Guangzhou CnFerment Biotechnology Co., Ltd., Guangzhou 510440, China.
Gram-negative bacteria possess an asymmetric outer membrane, where the outer leaflet consists of LPSs and the inner leaflet comprises phospholipids. , an opportunistic milk-borne pathogen that causes severe neonatal meningitis and bacteremia, displays diverse lipopolysaccharide (LPS) structures. As a barrier of the bacterial cell, LPSs likely influenced resistance to environment stresses; however, there are no research reports on this aspect, hindering the development of novel bactericidal strategies overcoming the pathogen's resilience.
View Article and Find Full Text PDFInfect Drug Resist
December 2024
Département de Biochimie Microbiologie, Ecole Doctorale Sciences Et Technologies (EDST)/Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso.
Purpose: The emergence of antibiotic resistance in pathogenic is a public health problem in tropical countries such as Burkina Faso. Antibiotic resistance could be identified using a variety of approaches. This study aimed to estimate the prevalence of pathogenic enterobacteria strains from three sources, as well as their antibiotic resistance profile to biotope and climatic season.
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