AI Article Synopsis
- The study aimed to create transgenic Tca8113 cells to examine the expression of the human endostatin (hES) gene in vitro.
- Researchers used lipofectamin to introduce the hES gene into Tca8113 cells, selecting successful transfectants with blasticidin S antibiotic.
- Results showed that the modified Tca8113 cells not only proliferated in the culture medium but also had a 100% expression rate of the hES gene.
Article Abstract
Objective: The purpose of this study was to establish transfergeneic Tca8113 cell and evaluate the expression of human endostatin (hES) gene in the cell colone in vitro.
Methods: To transfect hES gene into Tca8113 cells, lipofectamin was complexed with plasmid encoding hES gene, and blasticidin S antibiotic was adopted to select Tca8113--hES cell clone. Immunohistochemistry S-P method was adopted to detect the expression of hES in the transfergenic Tca8113 cell in vitro.
Results: Transfected by hES, the transfergenic Tca8113 cells could grow and proliferate in RPMI--1640 culture medium containing blasticidin S antibiotic. The expression rate of hES reached 100%.
Conclusion: hES gene can express in hES-transfected Tca8113 cell in vitro.
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