AI Article Synopsis
- The study aimed to obtain and express the full-length TRAIL gene in prokaryotic cells (E. coli).
- The TRAIL gene was amplified from peripheral blood cells using RT-PCR and cloned into the pGEX-2T vector for expression.
- The expressed TRAIL-GST demonstrated biological activity by inhibiting the proliferation of KB and Hela cells, leading to their apoptosis.
Article Abstract
Aim: To obtain and express the full-length of TRAIL gene in prokaryocytes.
Methods: The full-length of TRAIL gene was amplified from peripheral blood cells(PBCs)by RT-PCR and then cloned into the expression vector pGEX-2T. The TRAIL-GST was expressed in E.coli.
Results: Sequence analysis indicated that the sequence of TRAIL gene was identical with that in the GenBank. The TRAIL-GST was expressed in E.coli. Expressed product could inhibit the proliferation of KB and Hela cells and finally lead to their apoptosis.
Conclusion: The TRAIL-GST with biological activity has been expressed in the prokaryotic system.
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