AI Article Synopsis
- The study focuses on 7,8-dihydro-8-oxodeoxyguanosine (8-oxo-dG), a marker for oxidative DNA damage, using a specific antibody to detect it in marine organisms.
- Laboratory tests with benzo[a]pyrene (B[a]P) showed significant oxidative DNA damage in treated animals compared to controls.
- The immunohistochemical method allows for analysis without DNA extraction, using less tissue, and could help identify how different cell types respond to oxidative damage, making it useful in marine ecotoxicology.
Article Abstract
The modified nucleoside 7,8-dihydro-8-oxodeoxyguanosine (8-oxo-dG) is an index of oxidative DNA damage. An immunohistochemical approach based on the use of monoclonal antibody 1F7 against 8-oxo-dG was investigated in marine organisms with immunoperoxidase and immunofluorescent detection. Relative staining intensity as a measure of the 8-oxo-dG level was microscopically assessed. After laboratory exposures to benzo[a]pyrene (B[a]P), higher levels of oxidative DNA damage were clearly detected in all treated animals compared to controls. While this method eliminates DNA extraction reducing the processing of biological samples, absolute values are not provided. Further, the method requires only small amounts of tissue and potentially discriminates susceptibility to oxidative damage in different cell types. These results suggest that the assay should have practical applications in marine ecotoxicology.
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| http://dx.doi.org/10.1016/j.marenvres.2004.03.022 | DOI Listing |
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