AI Article Synopsis
- Supermacroporous acrylamide-based cryogels were created through a special polymerization process called cryo-polymerization, using specific monomers and a cross-linking agent.
- The resulting cryogels had a highly porous structure, with pore sizes ranging from 5 to 100 micrometers, and over 90% of their volume was made up of interconnected supermacropores filled with water.
- The ability of the cryogel columns to bind proteins remained consistent regardless of flow rates up to 600 cm/h, and the behavior of E. coli cells in ion-exchange processes was influenced by the cryogel's ligand density and porosity.
Article Abstract
Supermacroporous monolithic acrylamide (AAm)-based cryogels were prepared by radical cryo-polymerizaton (polymerization in the moderately frozen system) of AAm with functional monomers and cross-linker N,N'-methylene-bis-acrylamide (MBAAm). Electron microscopy studies revealed supermacroporous structure of the developed cryogels with pore size of 5-100 microm. Cryogel porosity depended on cryo-polymerization conditions. More than 90% of the monolithic bed volume is the interconnected supermacropores filled with water and less than 10% of the monolithic volume is pore walls. The total protein binding capacity (lysozyme in the case of immobilized metal affinity chromatography (IMAC) column and bovine serum albumin (BSA) in the case of anion-exchange (AE) column) was independent of the flow rates till 600 cm/h. Chromatographic behavior of E. coli cells when a cell suspension was applied to ion-exchange cryogel columns depended on both the density of functional ligand and the porosity of the cryogel.
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| http://dx.doi.org/10.1016/j.jchromb.2004.01.050 | DOI Listing |
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