In Arabidopsis leaves, chloroplast movement is fluence rate dependent. At optimal, lower light fluences, chloroplasts accumulate at the cell surface to maximize photosynthetic potential. Under high fluence rates, chloroplasts avoid incident light to escape photodamage. In this paper, we examine the phenomenon of chloroplast avoidance movement in greater detail and demonstrate a proportional relationship between fluence rate and the velocity of chloroplast avoidance. In addition we show that the amount of light-activated phototropin2, the photoreceptor for the avoidance response, likely plays a role in this phenomenon, as heterozygous mutant plants show a reduced avoidance velocity compared to that of homozygous wild type plants.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/b316285k | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Regulation of nucleus-encoded trans-acting factors allows orthogonal fine-tuning of multiple transgenes in the chloroplast of Chlamydomonas reinhardtii.
Plant Biotechnol J
December 2024
Department of Plant Sciences, University of Cambridge, Cambridge, UK.
The green microalga Chlamydomonas reinhardtii is a promising host organism for the production of valuable compounds. Engineering the Chlamydomonas chloroplast genome offers several advantages over the nuclear genome, including targeted gene insertion, lack of silencing mechanisms, potentially higher protein production due to multiple genome copies and natural substrate abundance for metabolic engineering. Tuneable expression systems can be used to minimize competition between heterologous production and host cell viability.
View Article and Find Full Text PDFArray of metabolic pathways in a kleptoplastidic foraminiferan protist supports chemoautotrophy in dark, euxinic seafloor sediments.
ISME J
January 2025
Department of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, United States.
Investigations of the metabolic capabilities of anaerobic protists advances our understanding of the evolution of eukaryotic life on Earth and for uncovering analogous extraterrestrial complex microbial life. Certain species of foraminiferan protists live in environments analogous to early Earth conditions when eukaryotes evolved, including sulfidic, anoxic and hypoxic sediment porewaters. Foraminifera are known to form symbioses as well as to harbor organelles from other eukaryotes (chloroplasts), possibly bolstering the host's independence from oxygen.
View Article and Find Full Text PDFPhototropin switches between cis- and trans-autophosphorylation in light-induced chloroplast relocation in Marchantia polymorpha.
Plant J
January 2025
Center for Bioscience Research and Education, Utsunomiya University, Tochigi, 321-8505, Japan.
In the accumulation response, chloroplasts move toward weak blue light (BL) to maximize photosynthetic efficiency; in the avoidance response, they move away from strong BL to reduce photodamage. The BL receptor kinase phototropin (phot) mediates these chloroplast relocation responses, and the chloroplast relocation response requires phot kinase activity. Upon receiving BL, phot undergoes autophosphorylation; however, the molecular mechanisms that regulate chloroplast relocation through phot autophosphorylation remain unclear.
View Article and Find Full Text PDFMolecular dynamics simulations of ribosome-binding sites in theophylline-responsive riboswitch associated with improving the gene expression regulation in chloroplasts.
J Bioinform Comput Biol
October 2024
Horticultural and Agricultural Research Department, Khuzestan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz 6134814538, Iran.
The existence of an efficient inducible transgene expression system is a valuable tool in recombinant protein production. The synthetic theophylline-responsive riboswitch (theo.RS) can be replaced in the 5[Formula: see text] untranslated region of an mRNA and control the translation of downstream gene in chloroplasts in response to the binding with a ligand molecule, theophylline.
View Article and Find Full Text PDFLight-regulated chloroplast morphodynamics in a single-celled dinoflagellate.
Proc Natl Acad Sci U S A
November 2024
Van der Waals-Zeeman Institute, Institute of Physics, University of Amsterdam, Amsterdam 1098XH, The Netherlands.
Photosynthetic algae play a significant role in oceanic carbon capture. However, their performance is constantly challenged by fluctuations in environmental light conditions. While phototaxis is a common strategy to cope with such fluctuations, nonmotile species must adopt alternative mechanisms to avoid light-induced damage.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!