This paper describes the application of a Köhler illuminated high-resolution wide-field microscope using surface plasmons to provide the image contrast. The response of the microscope to a grating structure in both the Fourier and the image planes is presented to demonstrate image formation by surface waves. The effect of spatial filtering in the back focal (Fourier) plane to enhance image contrast is described. We also discuss how the surface wave contrast mechanism affects the imaging performance of the microscope and discuss factors that can be expected to lead to even greater improvements in lateral resolution and sensitivity.
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http://dx.doi.org/10.1111/j.0022-2720.2004.01309.x | DOI Listing |
Nano Lett
January 2025
Department of Physics, Indian Institute of Technology Delhi, New Delhi 110016, India.
Structured illumination microscopy (SIM) is a robust wide-field optical nanoscopy technique. Several approaches are implemented to improve SIM's resolution capability (∼2-fold). However, achieving a high resolution with a large field of view (FOV) is still challenging.
View Article and Find Full Text PDFSingle-shot 3D optical microscopy that can capture high-resolution information over a large volume has broad applications in biology. Existing 3D imaging methods using point-spread-function (PSF) engineering often have limited depth of field (DOF) or require custom and often complex design of phase masks. We propose a new, to the best of our knowledge, PSF approach that is easy to implement and offers a large DOF.
View Article and Find Full Text PDFFourier ptychographic microscopy (FPM) enables high-resolution, wide-field imaging of both amplitude and phase, presenting significant potential for applications in digital pathology and cell biology. However, artifacts commonly observed at the boundaries of reconstructed images can significantly degrade imaging quality and phase retrieval accuracy. These boundary artifacts are typically attributed to the use of the fast Fourier transform (FFT) on non-periodic images.
View Article and Find Full Text PDFTransl Vis Sci Technol
December 2024
Department of Ophthalmology & Vision Sciences, University of Toronto, Toronto, ON, Canada.
Purpose: To demonstrate that high-seed, ultra-high-resolution spectral-domain optical coherence tomography (SD-OCT) technology can image in vivo fine morphological features in the healthy and pathological human limbus.
Methods: A compact, fiberoptic SD-OCT system was developed for imaging the human limbus. It combines ∼1.
Nanophotonics
July 2024
School of Electronics and Information Engineering, Sichuan University, Chengdu 610065, China.
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