Alkaline-heat-treated titanium self-forms an apatite surface layer in vivo. The aim of the present study was to materialistically characterize the surface of alkaline-heat-treated titanium immersed in simulated body fluid (AHS-TI) and to examine the differentiation behavior of osteoblasts on AHS-TI. SEM, thin-film XRD, FTIR, and XPS analyses revealed that AHS-TI contained a 1.0- micro m-thick, low-crystalline, and [002] direction-oriented carbonate apatite surface. Human osteoblast-like SaOS-2 cells were cultured on polystyrene, titanium, and AHS-TI, and RT-PCR analyses of osteogenic differentiation-related mRNAs were conducted. On AHS-TI, the expression of bone sialoprotein mRNA was up-regulated as compared with that on polystyrene and titanium (p < 0.05). On AHS-TI, the expression of osteopontin and osteocalcin mRNAs was up-regulated as compared with that on polystyrene (p<0.05). The results indicate that the apatite was bone-like and accelerated the osteogenic differentiation of SaOS-2, suggesting that alkaline-heat treatment might facilitate better integration of titanium implants with bone.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1177/154405910408300606 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Zinc (Zn) Doping by Hydrothermal and Alkaline Heat-Treatment Methods on Titania Nanotube Arrays for Enhanced Antibacterial Activity.
Nanomaterials (Basel)
May 2023
School of Advanced Materials Discovery, Colorado State University, Fort Collins, CO 80523, USA.
Titanium (Ti) is a popular biomaterial for orthopedic implant applications due to its superior mechanical properties such as corrosion resistance and low modulus of elasticity. However, around 10% of these implants fail annually due to bacterial infection and poor osseointegration, resulting in severe pain and suffering for the patients. To improve their performance, nanoscale surface modification approaches and doping of trace elements on the surfaces can be utilized which may help in improving cell adhesion for better osseointegration while reducing bacterial infection.
View Article and Find Full Text PDFA Novel Nanostructured Surface on Titanium Implants Increases Osseointegration in a Sheep Model.
Clin Orthop Relat Res
November 2022
Health and Biomedical Innovation, Clinical and Health Sciences, University of South Australia, Adelaide, Australia.
Background: A nanostructured titanium surface that promotes antimicrobial activity and osseointegration would provide the opportunity to create medical implants that can prevent orthopaedic infection and improve bone integration. Although nanostructured surfaces can exhibit antimicrobial activity, it is not known whether these surfaces are safe and conducive to osseointegration.
Questions/purposes: Using a sheep animal model, we sought to determine whether the bony integration of medical-grade, titanium, porous-coated implants with a unique nanostructured surface modification (alkaline heat treatment [AHT]) previously shown to kill bacteria was better than that for a clinically accepted control surface of porous-coated titanium covered with hydroxyapatite (PCHA) after 12 weeks in vivo.
The process of magnesium ion modification of titanium surface and the sustained-release of magnesium ions from its surface.
Dent Mater J
June 2020
Division of Dental Biomaterials, Tohoku University Graduate School of Dentistry.
This study explored modification of an alkaline heat treated titanium surface, using magnesium ions, to improve bone compatibility through the sustained release of magnesium ions. Pure titanium surface was first subjected to alkaline treatment using 5 M NaOH then modified with magnesium through immersion in magnesium chloride solution before heating in a furnace at 600°C for 1 h. Use of at least 0.
View Article and Find Full Text PDF[Study on biomimetic mineralization of lipopolysaccharide-amine nanopolymersomes/hyaluronic acid polyelectrolyte films on titanium surface].
Zhonghua Kou Qiang Yi Xue Za Zhi
February 2016
Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
Objective: To explore biomimetic mineralization of polyelectrolyte multilayer films (PEM) of gene-loaded lipopolysaccharide-amine nanopolymersomes/hyaluronic acid self assembled on titanium surface.
Methods: Via lay-by-layer self assembly technology, PEM were constructed on titanium or quartz surface using bone morphogenetic protein-2(BMP-2) plasmid-loaded lipopolysaccharide-amine nanopolymersomes(pLNP) as a polycation, and hyaluronic acid(HA) as a polyanion. The constructed PEM were defined as substrate-pLNP-(HA-pLNP)n, where a successive deposition of HA and pLNP on substrate surface was defined as one assembly cycle, and n was the cycle number.
[Study on construction and biological effects of lipopolysaccharide-amine nanopolymersomes/hyaluronic acid polyelectrolyte multilayer films on titanium surface].
Zhonghua Kou Qiang Yi Xue Za Zhi
December 2014
Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China. Email:
Objective: To provide a basis for surface modification of polyelectrolyte multilayer films (PEM) on implants by exploring the effects of immobilization of PEM on titanium surfaces on their cell biological effects.
Methods: By using plasmid of bone morphogenetic protein-2 (pBMP-2)-loaded lipopolysaccharide-amine nanopolymersomes (pLNP) as cationic polyelectrolytes and hyaluronic acid (HA) as anionic polyelectrolytes. PEM were constructed on alkaline-heat treated titanium surfaces via layer by layer self-assembly(LbL) technique.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!