The Butyrivibrio fibrisolvens tet(W) gene is located on the conjugative transposon TnB1230. TnB1230 encodes transfer proteins with 48 to 67% identity to some of those encoded by Tn1549. tet(W) is flanked by directly repeated sequences with significant homology to oxygen-insensitive nitroreductases. The 340 nucleotides upstream of tet(W) are strongly conserved and are required for tetracycline resistance.
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http://dx.doi.org/10.1128/JB.186.11.3656-3659.2004 | DOI Listing |
J Dairy Sci
December 2024
Department of Dairy and Food Sciences, South Dakota State University, Brookings, South Dakota 57007; School of Animal Sciences, Virginia Tech, Blacksburg, VA. Electronic address:
The objective of this study was to evaluate the effects of a rumen-derived direct-fed microbial (DFM) product on performance, blood biomarkers, ruminal fermentation, and bacterial abundance in dairy cows during the transition period until 100 DIM. Fifty-six Holstein cows were enrolled in a randomized complete block design from -21 to 100 DIM. Cows were blocked based on expected calving date, parity, and previous lactation milk yield for multiparous or genetic merit for primiparous cows.
View Article and Find Full Text PDFBMC Sports Sci Med Rehabil
December 2024
Department of Biological and Medical Science, Faculty of Physical Education and Sport, Comenius University in Bratislava, Bratislava, 814 69, Slovakia.
Background: Nowadays, obesity has become a major health issue. In addition to negatively affecting body composition and metabolic health, recent evidence shows unfavorable shifts in gut microbiota in individuals with obesity. However, the effects of weight loss on gut microbes and metabolites remain controversial.
View Article and Find Full Text PDFJ Agric Food Chem
December 2024
Swine Research Group, Agroscope, Posieux 1725, Switzerland.
Dietary tannins can affect rumen microbiota and enteric fermentation to mitigate methane emissions, although such effects have not yet been fully elucidated. We tested two subunits of hydrolyzable tannins named gallic acid (GA) and ellagic acid (EA), alone (75 mg/g DM each) or combined (150 mg/g DM in total), using the Rusitec system. EA and EA+GA treatments decreased methane production, volatile fatty acids, nutrient degradation, relative abundance of , , but increased .
View Article and Find Full Text PDFMicrobiol Resour Announc
October 2024
Department of Biological Sciences, California State University San Marcos, San Marcos, California, USA.
Here, we report the draft genome sequences of two -type strains isolated from rumen fluid. The genome sequence of DSM 14810 was 3.3 Mb with 3,093 predicted genes, while the DSM 3071 genome sequence was 4.
View Article and Find Full Text PDFAnaerobe
October 2024
Centre for Animal Nutrition and Welfare, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria.
Objectives: Feeding winery by-products (WBP) could affect the bovine microbiome because of their phenol compounds and a transfer of WBP-associated microbiota. This work examined changes in the underexplored solid-associated rumen microbiome following the inclusion of WBP.
Methods: Using the rumen simulation technique, fermenters were inoculated with the inoculum of donor cows and were fed one of six dietary treatments including a control diet of 70 % hay +30 % concentrate (CON), control diet + 3.
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