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Pan-cancer analysis combined with experimental validation revealed that is an immunological and prognostic biomarker.
Transl Cancer Res
November 2024
Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Background: Kinectin 1 () is a membrane protein involved in intracellular organelle motility. However, the role of in human pan-cancer lacks systematic analysis and evaluation. The aim of this study is to evaluate the expression profile and clinical value in human cancers by performing a pan-cancer analysis of .
View Article and Find Full Text PDFGeneration and characterization of zebrafish f9l mutant confirmed that f9l is f10 like gene.
Blood Coagul Fibrinolysis
December 2024
Department of Biological Sciences, University of North Texas, Denton, Texas, USA.
Aim: This study aimed to create an f9l mutant zebrafish using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) and characterize its coagulation properties to investigate its functional similarity to human FX and explore the potential synergy between f9l and f10.
Methods: Three gRNAs targeting exon 8 encoded by the catalytic domain of the f9l gene were injected into 300 single-cell zebrafish embryos using CRISPR/Cas9 technology. DNA from the resulting adults was extracted from tail tips, and PCR was used to detect indels.
Identification of a Flavanone 2-Hydroxylase Involved in Flavone -Glycoside Biosynthesis from .
J Agric Food Chem
December 2024
College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Tea contains a variety of flavone -glycosides, which are important compounds that distinguish tea cultivars and tea categories. However, the biosynthesis pathway of flavone -glycosides in tea plant remains unknown, and the key enzymes involved have not been characterized. In this study, a liquid chromatography-mass spectrometry method to determine 9 flavone -glycosides was developed, and the accumulation patterns of 9 flavone -glycosides in tea plants were examined first.
View Article and Find Full Text PDFA Study Modeling Bridged Nucleic Acid-Based ASOs and Their Impact on the Structure and Stability of ASO/RNA Duplexes.
Langmuir
October 2024
CMML-Catalysis and Molecular Modelling Lab, Department of Chemical Sciences, Tezpur University, Napaam, Sonitpur, Assam 784028, India.
Antisense medications treat diseases that cannot be treated using traditional pharmacological technologies. Nucleotide monomers of bare and phosphorothioate (PS)-modified LNA, N-MeO-amino-BNA, 2',4'-BNA[NH], 2',4'-BNA[NMe], and N-Me-aminooxy-BNA antisense modifications were considered for a detailed DFT-based quantum chemical study to estimate their molecular-level structural and electronic properties. Oligomer hybrid duplex stability is described by performing an elaborate MD simulation study by incorporating the PS-LNA and PS-BNA antisense modifications onto 14-mer ASO/RNA hybrid gapmer type duplexes targeting protein PTEN mRNA nucleic acid sequence (5'--3'/3'-GAAUCGUGACCGGA-5').
View Article and Find Full Text PDFA Methyl-Engineered DNAzyme for Endogenous Alkyltransferase Monitoring and Self-Sufficient Gene Regulation.
Small Methods
September 2024
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.
The on-demand gene regulation is crucial for extensively exploring specific gene functions and developing personalized gene therapeutics, which shows great promise in precision medicines. Although some nucleic acid-based gene regulatory tools (antisense oligonucleotides and small interfering RNAs) are devised for achieving on-demand activation, the introduction of chemical modifications may cause undesired side effects, thereby impairing the gene regulatory efficacy. Herein, a methyl-engineered DNAzyme (MeDz) is developed for the visualization of endogenous alkyltransferase (AGT) and the simultaneous self-sufficiently on-demand gene regulation.
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