AI Article Synopsis
- Analysis of crystal structures reveals that protein strands in serine protease active sites exhibit aligned atomic orbitals, which are crucial for their function.
- The active segments of enzymes like subtilisin BPN' and beta-trypsin show nearly identical orbital alignments, suggesting a shared evolutionary optimization for effective proteolysis.
- These findings indicate that the polarizability of protein strands enhances reaction rates for long oligopeptide substrates, making them more reactive even in non-enzymatic environments.
Article Abstract
By an analysis of PDB crystal structures, the mean conformations of protein strands bound in serine protease active sites are shown to contain extensively aligned atomic orbitals. The active-serine-bearing segment of each enzyme (subtilisin BPN' and beta-trypsin) also contains such alignments. The participating orbitals are almost identical in each system. All of the alignments converge on the targeted linkage. They suggest that a kind of through-strand polarizability is being optimized by evolution, presumably due to corresponding benefits in proteolysis rate. Such polarizability would help to explain the high values of kcat seen for long oligopeptide substrates. The idea predicts long substrates to be relatively reactive even under non-enzymatic conditions, which in fact they are.
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| http://dx.doi.org/10.1016/j.bmc.2004.02.044 | DOI Listing |
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